A lipocalin sequence signature modulates cell survival
A lipocalin sequence signature modulates cell survival
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DOI：
10.1016/j.febslet.2010.05.008
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发表时间：
2010-07-02
期刊：
影响因子：
3.5
通讯作者：
Reis, Cleyson V.
中科院分区：
文献类型：
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作者：
Chudzinski-Tavassi, Ana Marisa;Carrijo-Carvalho, Linda C.;Reis, Cleyson V.
脂质运载蛋白是β-桶蛋白，其氨基酸序列中共有三个保守基序。在这项研究中，我们确定了肽图谱的方法，7个氨基酸序列相关的这些基序之一（基序2），调节细胞存活。一种基于昆虫脂质运载蛋白的合成肽在血清缺乏的内皮细胞和白细胞中显示出细胞保护活性。这种活性依赖于一氧化氮合酶。该序列在几种脂质运载蛋白中发现，包括载脂蛋白D、视黄醇结合蛋白、脂质运载蛋白型前列腺素D合酶和许多未知蛋白，表明它是一个序列特征和脂质运载蛋白的保守性质。(C)2010年欧洲生物化学学会联合会。由Elsevier B出版。版权所有© 2016
Lipocalins are beta-barrel proteins, which share three conserved motifs in their amino acid sequence. In this study, we identified by a peptide mapping approach, a seven-amino acid sequence related to one of these motifs (motif 2) that modulates cell survival. A synthetic peptide based on an insect lipocalin displayed cytoprotective activity in serum-deprived endothelial cells and leucocytes. This activity was dependent on nitric oxide synthase. This sequence was found within several lipocalins, including apolipoprotein D, retinol binding protein, lipocalin-type prostaglandin D synthase, and many unknown proteins, suggesting that it is a sequence signature and a lipocalin conserved property. (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.