A Mycobacterium tuberculosis operon encoding ESAT-6 and a novel low-molecular-mass culture filtrate protein (CFP-10)
A Mycobacterium tuberculosis operon encoding ESAT-6 and a novel low-molecular-mass culture filtrate protein (CFP-10)
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DOI：
10.1099/00221287-144-11-3195
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发表时间：
1998-11-01
期刊：
影响因子：
--
通讯作者：
Gicquel, B
中科院分区：
文献类型：
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作者：
Berthet, FX;Rasmussen, PB;Gicquel, B
早期分泌的抗原性靶蛋白6 kDa（ESAT-6）是由结核分枝杆菌合成的有效T细胞蛋白抗原。其相应的基因（esat-6）位于RD 1中，RD 1是减毒结核疫苗株牛分枝杆菌BCC中缺失的10 kb DNA区域。M.结核杆菌ESAT-6的克隆和鉴定。鉴定了一个与esat-6共转录的新基因，命名为Ihp。此外，计算机搜索在M。结核病基因组鉴定了13个与Ihp/esat-6操纵子相关的基因，定义了一个新的基因家族。利用M.结核RNA。相应的启动子信号在耻垢分枝杆菌中不被识别，其中Ihp/esat-6的转录起始于不同的位置。分枝结核Ihp基因产物被鉴定为CFP-10，一种在短期培养滤液中发现的低分子量蛋白。这些结果表明，编码CFP-10和ESAT-6的基因在M.结核病和这两个编码小的输出蛋白质。
The early secreted antigenic target 6 kDa protein (ESAT-6) is a potent T-cell protein antigen synthesized by Mycobacterium tuberculosis. Its corresponding gene (esat-6) is located in RD1, a 10 kb DNA region deleted in the attenuated tuberculosis vaccine strain Mycobacterium bovis BCC. The promoter region of M. tuberculosis esat-6 was cloned and characterized. A new gene, designated Ihp and cotranscribed with esat-6 was identified. Moreover, computer searches in the M. tuberculosis genome identified 13 genes related to the Ihp/esat-6 operon, defining a novel gene family. The transcription initiation sites of the Ihp/esat-6 operon were mapped using M. tuberculosis RNA. The corresponding promoter signals were not recognized in Mycobacterium smegmatis, in which transcription of Ihp/esat-6 is initiated at different locations. The M. tuberculosis Ihp gene product was identified as CFP-10, a low-molecular-mass protein found in the short-term culture filtrate. These results show that the genes encoding CFP-10 and ESAT-6 are transcribed together in M. tuberculosis and that both code for small exported proteins.