Detection and genetic analysis of a novel atypical porcine pestivirus from piglets with congenital tremor in Japan
Detection and genetic analysis of a novel atypical porcine pestivirus from piglets with congenital tremor in Japan
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DOI：
10.1111/tbed.14149
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发表时间：
2021-05-29
影响因子：
4.3
通讯作者：
Nagai, Makoto
中科院分区：
文献类型：
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作者：
Kasahara-Kamiie, Miwako;Kagawa, Mitsuo;Nagai, Makoto
非典型猪瘟病毒（APPV）是一种新发现的猪病毒，已被证实与猪的先天性震颤（CT）有关，在美洲、欧洲和亚洲均有发现，但在日本尚未有APPV的报道。本研究利用CT技术在日本仔猪中枢神经系统中鉴定出一株APPV，并首次测定和分析了其全基因组序列。利用日本APPV（命名为安娜/2020）的全基因组核苷酸序列和NCBI数据库中的APPV的全基因组核苷酸序列进行系统发育分析，结果表明APPV分为3种基因型（基因型1至3），并且安娜/2020与基因型3的APPV株聚在一起，但与这些株远距离分支。基因3型毒株之间的同源性为94.0%~ 99.7%，而安娜/2020与基因3型毒株的同源性为87.0%~ 89.3%，表明安娜/2020代表了基因3型中一个新的APPV谱系。使用RT-PCR进行的回顾性检查显示，来自无CT猪的1种基因型1和2种新型基因型3 APPV，并且至少自2007年以来新型基因型3 APPV在日本流行。
Atypical porcine pestivirus (APPV), which has been confirmed to be associated with congenital tremor (CT) in pigs, is a newly discovered porcine virus that has been found in the Americas, Europe and Asia; however, no report of APPV in Japan has been published. We identified an APPV in the central nervous system of Japanese piglets with CT and firstly determined and analysed the complete genome sequence. Phylogenetic analysis using the complete genome nucleotide sequence of the Japanese APPV, named Anna/2020, and those of APPVs from the NCBI database showed that APPVs were divided into three genotypes (genotypes 1 to 3), and that Anna/2020 clustered with the genotype 3 APPV strains, but distantly branched from these strains. Pairwise complete coding region nucleotide sequence comparisons revealed that there was 94.0%- 99.7% sequence identity among the genotype 3 strains, while Anna/2020 showed 87.0%-89.3% identity to those genotype 3 strains, suggesting that Anna/2020 represents a novel APPV lineage within genotype 3. Retrospective examinations using RT-PCR revealed one genotype 1 and two novel genotype 3 APPVs from pigs without CT, and that novel genotype 3 APPVs have been prevalent in Japan since at least 2007.