Supercritical fluid extraction from rosemary (Rosmarinus officinalis):: Kinetic data, extract's global yield, composition, and antioxidant activity
Supercritical fluid extraction from rosemary (Rosmarinus officinalis):: Kinetic data, extract's global yield, composition, and antioxidant activity
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DOI：
10.1016/j.supflu.2005.01.009
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发表时间：
2005-10-01
影响因子：
3.9
通讯作者：
Meireles, MAA
中科院分区：
文献类型：
--
作者：
Carvalho, RN;Moura, LS;Meireles, MAA
本文测定了迷迭香+CO2体系的总产率等温线和总提取曲线，以及提取物的化学组成和抗氧化活性。实验在两套超临界萃取装置上进行。床高与床直径的比率如下：0.67、1.34、2.8、5.6和8.4。在100-300巴的压力下，确定了30摄氏度和40摄氏度的等温线的全球产率。总的萃取曲线在40℃和300bar下测定。迷迭香提取物用水蒸馏(挥发油)，用溶剂、乙醇和正己烷提取。用气相色谱法测定提取物的化学成分，用紫外分光光度法测定酚类二萜类化合物(鼠尾草酸和迷迭香酸)的含量。通过β-胡萝卜素与亚麻酸的偶联反应测定其抗氧化活性。在40摄氏度和300巴时，获得了最大的全球产率(5%干基)。提取物中的主要成分为樟脑和1，8-桉树酚。在150巴和30℃下获得的提取物的长期抗氧化活性从80%到300巴和40℃下获得的提取物的95%不等。在300巴和40℃下获得的提取物的鼠尾酸含量是挥发油的9倍。Crank，Esquivel等人，Goto等人，Martinez等人，Sovova，Tan和Liou等人的模型定量地拟合了整个提取曲线。然而，Martinez等人、Crank、Tan和Lion等人的模型的均方偏差大约是Goto等人、Sovova和Esquivel等人的模型的五倍。(C)2005 Elsevier B.V.保留所有权利。
In the present work the global yields isotherms and the overall extraction curves for the system rosemary (Rosmarinus officinalis) + CO2, the chemical composition and antioxidant activity of the extracts were determined. The experiments were performed in two supercritical extraction units. The following bed height to bed diameter ratios were used: 0.67, 1.34, 2.8, 5.6, and 8.4. The global yields were determined for the isotherms of 30 and 40 degrees C for pressures of 100-300 bar. The overall extraction curves were determined at 40 degrees C and 300 bar. Rosemary extracts were also obtained by hydrodistillation (volatile oil) and extraction with solvent, ethanol and hexane were used. The chemical compositions of the extracts were determined by gas chromatography; the contents of phenolic diterpenes (carnosoic and rosmarinic acids) were determined by UV spectrophotometry. The antioxidant activity was measured by the coupled reaction of beta-carotene and linolenic acid. The largest global yield (5% dry basis) was obtained at 40 degrees C and 300 bar. The major compounds detected in the extracts were camphor and 1,8-cineol. The long term antioxidant activity varied from 80% for the extract obtained at 150 bar and 30 degrees C to 95% for the extract obtained at 300 bar and 40 degrees C. The content of carnosoic acid was nine times larger for the extract obtained at 300 bar and 40 degrees C as compared to the volatile oil. The models of Crank, Esquivel et al., Goto et al., Martinez et al., Sovova, and Tan and Liou quantitatively fitted the overall extraction curves. Nonetheless, the mean square deviations for the models of Martinez et al., Crank, and Tan and Lion were approximately five times larger than that for the models of Goto et al., Sovova, and Esquivel et al. (c) 2005 Elsevier B.V. All rights reserved.