Research article -: The genome of the sea urchin Strongylocentrotus purpuratus
Research article -: The genome of the sea urchin Strongylocentrotus purpuratus
复制标题
DOI：
10.1126/science.1133609
复制
发表时间：
2006-11-10
期刊：
影响因子：
56.9
通讯作者：
Wright, Rita
中科院分区：
文献类型：
--
作者：
Sodergren, Erica;Weinstock, George M.;Wright, Rita
我们报道了海胆（一种发育和系统生物学模型）814兆碱基的基因组序列和分析。采用全基因组霰弹枪和细菌人工染色体（BAC）序列相结合的测序策略。这种BAC克隆的使用，在汇集策略的帮助下，克服了与基因组高杂合性相关的困难。基因组编码了大约23,300个基因，其中包括许多以前被认为是脊椎动物创新的基因，或者只在后口动物之外才知道的基因。这个棘皮动物基因组为脊索动物提供了一个进化外群，并为后口动物的进化提供了见解。
We report the sequence and analysis of the 814-megabase genome of the sea urchinStrongylocentrotus purpuratus, a model for developmental and systems biology. The sequencing strategy combined whole-genome shotgun and bacterial artificial chromosome (BAC) sequences. This use of BAC clones, aided by a pooling strategy, overcame difficulties associated with high heterozygosity of the genome. The genome encodes about 23,300 genes, including many previously thought to be vertebrate innovations or known only outside the deuterostomes. This echinoderm genome provides an evolutionary outgroup for the chordates and yields insights into the evolution of deuterostomes.