RED PHOTON TREATMENT INHIBITS APOPTOSIS VIA REGULATION OF BCL-2 PROTEINS AND ROS LEVELS, ALLEVIATING HYPOXIC-ISCHEMIC BRAIN DAMAGE
RED PHOTON TREATMENT INHIBITS APOPTOSIS VIA REGULATION OF BCL-2 PROTEINS AND ROS LEVELS, ALLEVIATING HYPOXIC-ISCHEMIC BRAIN DAMAGE
复制标题
红色光子治疗通过调节 bcl-2 蛋白和 ros 水平抑制细胞凋亡，减轻缺氧缺血性脑损伤
DOI：
10.1016/j.neuroscience.2014.02.034
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发表时间：
2014-05-30
期刊：
影响因子：
3.3
通讯作者：
Xiao, N.
中科院分区：
文献类型：
--
作者：
Jiang, W.;Chen, L.;Xiao, N.
缺氧缺血性脑损伤（HIBD）的治疗选择是稀缺和低效的。近年来的研究表明，红光子在HIBD的抗炎和凋亡过程中发挥着重要作用。本研究探讨了红光子对SD大鼠HIBD和PC 12细胞氧糖剥夺（OGD）的保护作用。检测PC 12细胞凋亡、线粒体跨膜电位（MMP）和活性氧（ROS）的变化。我们发现，6小时的照射导致MMP，ROS和凋亡率下降，虽然这些变化是可逆的延长照射。重要的是，这些效应在红色光子猝灭后持续2-8小时。Bax和bcl-2蛋白和mRNA表达也有类似的变化趋势，短期照射（6 h）可抑制PC 12细胞凋亡。然而，长时间（> 6 h）照射引起细胞损伤。在体内实验中，bax的mRNA和蛋白水平降低7天后，在HIBD模型大鼠用红光子治疗，与bcl-2。bax和bcl-2主要表达于海马CA 1和CA 3区锥体细胞。重要的是，Morris水迷宫测试结果显示照射后大鼠的学习能力和空间记忆得到改善。总之，我们的数据表明，在急性期用红光子短期照射通过调节bcl-2相关蛋白和降低ROS水平来抑制线粒体凋亡途径，从而减少神经细胞的凋亡，改善HIBD的神经预后。(C)2014年IBRO。由爱思唯尔有限公司出版。保留所有权利。
Therapeutic options for hypoxic-ischemic brain damage (HIBD) are scarce and inefficient. Recently, many studies have demonstrated that red photon plays an important role in anti-inflammatory processes as well as apoptosis, the main trait of HIBD. In this study, we investigated whether red photon can protect from HIBD in SD rats and oxygen-glucose deprivation (OGD) in PC12 cells. Apoptosis, mitochondrial transmembrane potential (MMP), and reactive oxygen species (ROS) rates were assessed in PC12 cells. We found that 6-h irradiation resulted in decreased MMP, ROS and apoptosis rates, although these changes were reversible with prolonged irradiation. Importantly, these effects were sustained for 2-8 h upon quenching of the red photon. Similar trends were observed for protein and mRNA expression of bax and bcl-2, with shortterm irradiation (6 h) inhibiting apoptosis in PC12 Cells. However, long-term (> 6 h) irradiation caused cell damage. In vivo experiments, bax mRNA and protein levels were reduced after 7 days in HIBD model rats treated with red photon, in contrast to bcl-2. Furthermore, we found that bax and bcl-2 were mainly expressed in pyramidal cells of the hippocampus CA1 and CA3. Importantly, Morris Water Maze test results revealed an improvement in learning ability and spatial memory in rats after irradiation. Overall, our data showed that short-term irradiation with red photon in the acute phase inhibits the mitochondrial apoptotic pathway via regulation of bcl-2-related proteins and reduction of ROS levels, thereby decreasing apoptosis in nerve cells and improving the neurological prognosis of HIBD. (C) 2014 IBRO. Published by Elsevier Ltd. All rights reserved.