Occurrence and molecular characterization of Turkish isolates of Turnip mosaic virus
Occurrence and molecular characterization of Turkish isolates of Turnip mosaic virus
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DOI：
10.1111/j.1365-3059.2008.01902.x
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发表时间：
2008-12
期刊：
影响因子：
2.7
通讯作者：
S. Korkmaz;Y. Tomitaka;S. Onder;K. Ohshima
中科院分区：
文献类型：
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作者：
S. Korkmaz;Y. Tomitaka;S. Onder;K. Ohshima
2004-06 年生长季节，从土耳其西南部马莫拉地区种植十字花科和非十字花科作物的田地中收集了总共 142 个表现出芜菁花叶病毒 (TuMV) 症状的植物样本。采用酶联免疫吸附试验（ELISA）在土耳其主要芸苔作物田中检测到TuMV，总发病率为13·4%。在抱子甘蓝、卷心菜、野芥菜、萝卜和野萝卜的样品中检测到 TuMV，但在花椰菜或西兰花中未检测到。确定了两个生物学上不同的分离株 TUR1 和 TUR9 的基因组 RNA 的全长序列。重组分析表明，TUR1 是谱系内重组体，而 TUR9 是非重组体。对土耳其分离株与世界其他地区分离株的系统发育分析表明，TUR1 和 TUR9 分离株分别属于世界芸苔属和亚洲芸苔属/萝卜属群体。这项研究表明TuMV广泛分布在土耳其小亚细亚地区。
A total of 142 samples of plants showing symptoms of Turnip mosaic virus (TuMV) were collected from fields planted to Brassicaceae and non-Brassicaceae crops in the southwest Marmora region of Turkey, during the 2004−06 growing seasons. Using enzyme-linked immunosorbent assay (ELISA) TuMV was detected in the main brassica-crop fields of Turkey, with an overall incidence of 13·4%. TuMV was detected in samples from Brussels sprouts, cabbage, wild mustard, radish and wild radish, but not cauliflower or broccoli. The full-length sequences of the genomic RNAs of two biologically distinct isolates, TUR1 and TUR9, were determined. Recombination analyses showed that TUR1 was an intralineage recombinant, whereas TUR9 was a non-recombinant. Phylogenetic analyses of the Turkish isolates with those from the rest of the world showed that the TUR1 and TUR9 isolates belonged to world-Brassica and Asian-Brassica/Raphanus groups, respectively. This study showed that TuMV is widely distributed in the Asia Minor region of Turkey.