RELATIONSHIP BETWEEN TOTAL MAGNESIUM CONCENTRATION AND FREE INTRACELLULAR MAGNESIUM IN SHEEP RED-BLOOD-CELLS
RELATIONSHIP BETWEEN TOTAL MAGNESIUM CONCENTRATION AND FREE INTRACELLULAR MAGNESIUM IN SHEEP RED-BLOOD-CELLS
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DOI：
10.1016/0167-4889(91)90025-s
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发表时间：
1991-08-13
期刊：
影响因子：
--
通讯作者：
LAUF, PK
中科院分区：
文献类型：
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作者：
FUJISE, H;CRUZ, P;LAUF, PK
用P-31核磁共振法测定了离子载体A23187透化高钾绵羊红细胞的游离镁浓度，用原子吸收法测定了细胞总镁浓度。 游离镁浓度与总细胞镁浓度在0.3至1.92 mmol Mg/L细胞的浓度范围内产生对数-对数标度的线性关系。 因此，细胞内游离镁浓度可以从原子吸收数据计算。 该方法允许估计生理或实验诱导的细胞内游离镁浓度在细胞水中7和405 μ M镁之间的变化。 该范围包括未处理红细胞测定的细胞水中游离镁浓度335 +/- 60 μ M。
The cellular free magnesium concentration of ionophore A23187 permeabilized high potassium sheep erythrocytes was measured by P-31 nuclear magnetic resonance spectroscopy, and the total cellular magnesium concentration was determined by atomic absorption spectroscopy. The free versus total cellular magnesium concentrations yield a linear relationship on a log-log scale in the concentration range from 0.3 to 1.92 mmol Mg/liter cells. Thus, free intracellular magnesium concentrations can be calculated from atomic absorption data. The method permits the estimation of physiologically or experimentally induced variations of intracellular free magnesium concentrations between 7 and 405-mu-M magnesium in cell water. This range encompasses the free magnesium concentration of 335 +/- 60-mu-M in cell water determined for untreated erythrocytes.