CLONING AND CHARACTERIZATION OF A VASOPRESSIN V2 RECEPTOR AND POSSIBLE LINK TO NEPHROGENIC DIABETES-INSIPIDUS
CLONING AND CHARACTERIZATION OF A VASOPRESSIN V2 RECEPTOR AND POSSIBLE LINK TO NEPHROGENIC DIABETES-INSIPIDUS
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DOI：
10.1038/357336a0
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发表时间：
1992-05-28
期刊：
影响因子：
64.8
通讯作者：
BROWNSTEIN, MJ
中科院分区：
文献类型：
--
作者：
LOLAIT, SJ;OCARROLL, AM;BROWNSTEIN, MJ
精氨酸加压素（AVP）的抗利尿作用是由连接腺苷酸环化酶1的肾型（V2）受体介导的。我们在这里报告的大鼠肾脏V2 AVP受体互补DNA的克隆，编码一个370个氨基酸的蛋白质与跨膜地形特征的G蛋白偶联受体，并与V1 A（肝）AVP受体2在其7个跨膜域的相似性。克隆的cDNA在哺乳动物细胞中的表达显示出特异性配体结合和天然V2 AVP受体的活性特征。受体信使RNA仅在肾脏中检测到。人V2受体基因已定位于X染色体的长臂，靠近肾源性尿崩症的基因座，这是一种X连锁隐性疾病，其特征在于对AVP 3-6的抗利尿作用的肾抗性。
THE antidiuretic effect of arginine vasopressin (AVP) is mediated by renal-type (V2) receptors linked to adenylyl cyclase 1. We report here the cloning of the rat kidney V2 AVP receptor complementary DNA that encodes a 370-amino-acid protein with a transmembrane topography characteristic of G protein-coupled receptors, and with similarity to the V1a (hepatic) AVP receptor 2 in its seven membrane-spanning domains. Expression of the cloned cDNA in mammalian cells showed specific ligand binding and activity characteristic of the native V2 AVP receptor. The receptor messenger RNA is detected only in the kidney. The human V2 receptor gene has been localized to the long arm of the X chromosome close to the locus for nephrogenic diabetes insipidus, an X-linked recessive disorder characterized by renal resistance to the antidiuretic action of AVP 3-6.