COMPARATIVE SENSITIVITY OF SURVIVAL-ADJUSTED CHI-SQUARE AND NORMAL STATISTICS FOR THE MUTAGENESIS FLUCTUATION ASSAY
COMPARATIVE SENSITIVITY OF SURVIVAL-ADJUSTED CHI-SQUARE AND NORMAL STATISTICS FOR THE MUTAGENESIS FLUCTUATION ASSAY
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DOI：
10.1002/jat.2550060206
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发表时间：
1986-04-01
影响因子：
3.3
通讯作者：
MOORE, CM
中科院分区：
文献类型：
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作者：
GOODALL, CM;STEPHENS, OB;MOORE, CM
通过模拟和二甲基亚硝胺、二乙基硝胺的酶激活试验，对微量平板诱变波动试验的三个统计量进行了研究。将生存调整的.chi2统计量(‘’Gsq‘’)与卡茨‘’S正态分布统计量(‘’Phi‘’)以及Gilbert的生存独立统计量(‘’Zsq‘’)进行比较。当毒性很低或很高时，phi统计要么不能在可能的背景突变频率的整个范围内进行评估，要么有时它表明统计意义的异常高水平，即使其他测试是阴性的。在毒性和突变背景值的实验有用范围内，经生存调整的GSQ密切遵循ZSQ统计数据，并在敏感度方面有所改善。在80。+-范围内。生存率约10%，卡茨“S统计”“Phi”是最敏感的。统计检验的选择可能会对最小有效诱变浓度的估计产生10-100倍的影响。对于筛查未知数，两种类型的检测(Phi和Gsq(或Zsq))都可能有助于检测可疑的前诱变剂和设计验证性试验。需要细菌种群统计来评估统计阳性结果的价值。
Three statistics for analysis of microtitre plate mutagenesis fluctuation tests were studied by simulation, and in enzyme-activated assays of dimethylnitrosamine and diethylnitramine. A survival-adjusted .chi.2 statistic (''Gsq'') was compared with Katz''s normally distributed statistic (''Phi''), and with the survival-independent statistic (''Zsq'') of Gilbert. When toxicity was either very low or high, the Phi statistic either could not be evaluated over the whole range of possible background mutant frequencies, or sometimes it indicated unusually high levels of statistical significance, even when the other tests were negative. The survival-adjusted Gsq closely followed the Zsq statistic throughout the experimentally useful range of toxicities and mutant background values, with some improvement in sensitivity. Within the range 80 .+-. 10% survival approximately, Katz''s statistic ''Phi'' was the most sensitive. The choice of statistical test could affect the estimate of the minimal effective mutagenic concentration by a factor of 10-100. For screening unknowns, both types of test (Phi and Gsq (or Zsq)) may help in detecting suspect pro-mutagens and in designing a confirmatory assay. Bacterial population statistics are needed to assess the value of statistically positive results.