CD4+CD25+ regulatory T cells (TREG) in Systemic Lupus Erythematosus (SLE) patients:: The possible influence of treatment with corticosteroids
CD4+CD25+ regulatory T cells (TREG) in Systemic Lupus Erythematosus (SLE) patients:: The possible influence of treatment with corticosteroids
复制标题
DOI：
10.1016/j.clim.2007.12.010
复制
发表时间：
2008-05-01
影响因子：
8.6
通讯作者：
Mashahit, M. A.
中科院分区：
文献类型：
--
作者：
Azab, N. A.;Bassyouni, I. H.;Mashahit, M. A.
系统性红斑狼疮（SLE）是一种慢性全身性自身免疫性疾病，其特征是对自身抗原的耐受性丧失。调节性T细胞（T-REG）是组成性表达高水平的CD 25并表现出强大的抑制特性的那些CD 4 + T细胞。本研究的目的是定量SLE患者外周血中的CD 4 + CD 25+（T-REG）细胞和TREG的平均荧光指数（MFI），并将这些结果与疾病活动评分和药物治疗相关联。本研究包括24例SLE患者的各种疾病活动评分（SLEDAI）和24名健康的年龄和性别匹配的对照。流式细胞术检测CD 4 + CD 25 + T细胞的频率和CD 4 + CD 25+（高）T细胞的MFI（T-REG）。SLE患者外周血CD 4 + CD 25 + T细胞百分率及CD 4 + CD 25+（high）T细胞MFI均高于对照组CD 4 + CD 25 + T细胞百分比和CD 4 + CD 25+（high）T细胞的MFI与SLEDAI评分呈高度显著相关（P值分别为0.62和0.037（均P < 0.001），并且在服用糖皮质激素的患者中高于未服用糖皮质激素的患者（分别P = 0.023、0.048）。我们的结论是，我们的患者中Treg细胞的增加可能是由于皮质类固醇治疗。(C)2008年爱思唯尔公司All rights reserved.
Systemic Lupus Erythematosus (SLE) is a chronic, systemic autoimmune disease characterized by toss of tolerance to self-antigens. Regulatory Tcells (T-REG) are those CD4+ Tcells that constitutively express high levels of CD25 and exhibit powerful suppressive properties. The aim of this work was to quantify CD4+CD25+ (T-REG) cells and the Mean Fluorescence Index (MFI) of TREG in the peripheral blood of patients with SLE and to correlate these findings with their disease activity scores and drug therapy. This study included 24 SLE patients with various disease activity scores (SLEDAI) and 24 healthy age and sex matched controls. Flow cytometry was used to examine the frequency of CD4+CD25+ T cells and the MFI of CD4+CD25+(high) T cells (T-REG). CD4+ CD25+ T cells % and MFI of CD4+CD25+(high) T cells were higher in SLE patients than controls (p value = 0.62 and = 0.037 respectively) and both CD4+CD25+ T cell % and the MFI of CD4+CD25+(high) T cells showed highly significant correlation with SLEDAI scores (both with a p value < 0.001) and were higher in patients taking glucocorticoids than those not on glucocorticoids (p = 0.023, 0.048 respectively). We conclude that the increase in TREG cells in our patients may be due to corticosteroid treatment. (C) 2008 Elsevier Inc. All rights reserved.