A COMPARATIVE-STUDY OF CHARGE MOVEMENT IN RAT AND FROG SKELETAL-MUSCLE FIBERS
A COMPARATIVE-STUDY OF CHARGE MOVEMENT IN RAT AND FROG SKELETAL-MUSCLE FIBERS
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DOI：
10.1113/jphysiol.1981.sp014004
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发表时间：
1981-01-01
影响因子：
5.5
通讯作者：
MARSHALL, MW
中科院分区：
文献类型：
--
作者：
HOLLINGWORTH, S;MARSHALL, MW
纤维电压钳技术(禤浩焯和马歇尔)的中间部分被用于测量哺乳动物快抽动(大鼠指长伸肌)、哺乳动物慢抽动(大鼠比目鱼肌)和青蛙(缝匠肌)肌肉的非线性电荷运动。哺乳动物快抽动肌在2度时运动的最大电荷量。C在高渗液中是慢肌的3-5倍。快抽动充电的电压分布比慢抽动多10-15 mV。在这两种类型的哺乳动物肌肉中，高渗林格溶液使电荷的电压分布负移了约6 mV。电荷在机械阈值附近移动的陡度不受高渗性的影响。青蛙缝纫肌纤维在2度时的电荷量。C在高渗溶液中为.apprx。大鼠快速抽动肌的电压分布与大鼠比目鱼肌相似。气温上升了2到15度。C对哺乳动物或青蛙肌肉中电荷的数量或稳态分布都没有影响。在2度。在相同温度下，快、慢抽动哺乳动物肌肉的电荷运动动力学相似，比蛙肌快2-3倍。在2度的快和慢的哺乳动物纤维中。C个相似的时间来移动电荷总量的相同分数。所研究的3块肌肉的电荷运动动力学Q10介于1.2和2.0之间。
The middle of the fiber voltage-clamp technique (Adrian and Marshall), modified where necessary for electrically short muscle fibers, was used to measure non-linear charge movements in mammalian fast twitch (rat extensor digitorum longus), mammalian slow twitch (rat soleus) and frog (sartorius) muscles. The maximum amount of charge moved in mammalian fast twitch muscle at 2.degree. C in hypertonic solution, was 3-5 times greater than in slow twitch muscle. The voltage distribution of fast twitch charge was 10-15 mV more positive when compared to slow twitch. In both mammalian muscle types hypertonic Ringer solution negatively shifted the voltage distribution of charge some 6 mV. The steepness of charge moved around mechanical threshold was unaffected by hypertonicity. The amount of charge in frog sartorius fibers at 2.degree. C in hypertonic solution was .apprx. 1/2 of that in rat fast twitch muscle; the voltage distribution of the frog charge was similar to rat soleus muscle. Warming between 2 and 15.degree. C had no effect on either the amount or steady-state distribution of charge in mammalian or frog muscles. At 2.degree. C the kinetics of charge movement in fast and slow twitch mammalian muscles were similar and 2-3 times faster than frog muscle at the same temperature. In fast and slow mammalian fibers at 2.degree. C similar times were taken to shift the same fractions of the total amount of charge. The Q10 of charge movement kinetics was between 1.2 and 2.0 in the 3 muscles studied.