Motor-like DNA motion due to an ATP-hydrolyzing protein under nanoconfinement.
Motor-like DNA motion due to an ATP-hydrolyzing protein under nanoconfinement.
复制标题
纳米限制下 ATP 水解蛋白产生类似马达的 DNA 运动。
DOI：
10.1038/s41598-018-28278-0
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发表时间：
2018
影响因子：
4.6
通讯作者：
Riehn,Robert
中科院分区：
文献类型：
--
作者：
Roushan,Maedeh;Azad,Zubair;Movahed,Saeid;Ray,PaulD;Livshits,GideonI;Lim,ShuangFang;Weninger,KeithR;Riehn,Robert
我们报道了限制在准一维纳米通道中的长双链DNA在T4 DNA连接酶的作用下在必要的辅助因子的存在下进行超扩散运动。在纳米通道的受限环境中，双链DNA分子由于自我避免相互作用而伸展。在没有催化活性酶的情况下，我们看到的是经典的质心扩散。然而，蛋白质与DNA的合作相互作用可以导致DNA分子在纳米通道内的定向运动。在这里，我们展示了三种不同蛋白质(T4 DNA连接酶，MutS，E。coliDNA连接酶)在它们的能量辅助因子（ATP, NAD+）的存在下。
We report that long double-stranded DNA confined to quasi-1D nanochannels undergoes superdiffusive motion under the action of the enzyme T4 DNA ligase in the presence of necessary co-factors. Inside the confined environment of the nanochannel, double-stranded DNA molecules stretch out due to self-avoiding interactions. In absence of a catalytically active enzyme, we see classical diffusion of the center of mass. However, cooperative interactions of proteins with the DNA can lead to directed motion of DNA molecules inside the nanochannel. Here we show directed motion in this configuration for three different proteins (T4 DNA ligase, MutS,E. coliDNA ligase) in the presence of their energetic co-factors (ATP, NAD+).