Ser-127-to-Leu substitution in the DNA gyrase B subunit of Streptococcus pneumoniae is implicated in novobiocin resistance
Ser-127-to-Leu substitution in the DNA gyrase B subunit of Streptococcus pneumoniae is implicated in novobiocin resistance
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DOI：
10.1128/jb.177.14.4166-4170.1995
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发表时间：
1995-07
影响因子：
3.2
通讯作者：
R. Muñoz;M. Bustamante;A. G. de la Campa
中科院分区：
文献类型：
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作者：
R. Muñoz;M. Bustamante;A. G. de la Campa
我们报告的gyrB基因从肺炎链球菌533携带nov-1等位基因的克隆。gyrB基因编码与古细菌和真细菌的促旋酶B亚基同源的蛋白质。相同的氨基酸替换（Ser-127到Leu）赋予新生霉素抗性于四个分离的S.肺炎。该氨基酸位置相当于大肠杆菌GyrB的瓦尔-120，如蛋白质的晶体结构所示，该残基位于ATP结合结构域内。
We report the cloning of the gyrB gene from Streptococcus pneumoniae 533 that carries the nov-1 allele. The gyrB gene codes for a protein homologous to the gyrase B subunit of archaebacteria and eubacteria. The same amino acid substitution (Ser-127 to Leu) confers novobiocin resistance on four isolates of S. pneumoniae. This amino acid position is equivalent to Val-120 of Escherichia coli GyrB, a residue that lies inside the ATP-binding domain as revealed by the crystal structure of the protein.