Reversed end Ds element: a novel tool for chromosome engineering in Arabidopsis
Reversed end Ds element: a novel tool for chromosome engineering in Arabidopsis
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DOI：
10.1007/s11103-008-9377-6
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发表时间：
2008-11-01
影响因子：
5.1
通讯作者：
Peterson, Thomas
中科院分区：
文献类型：
--
作者：
Krishnaswamy, Lakshminarasimhan;Zhang, Jianbo;Peterson, Thomas
玉米Ac/Ds转座因子（TE）通过“剪切和粘贴”机制转座。先前在玉米中的研究表明，当TE末端相对于彼此处于反向取向时，可发生替代性转座反应，导致大规模基因组重排，包括缺失和倒位。为了测试类似的基因组重排是否也可以发生在其他植物中，我们研究了这种替代转座介导的基因组重排在拟南芥中的功效。在这里，我们提出了我们的分析33个独立的染色体重排。Ds元件反端的转座可引起超过1 Mbp的缺失和高达2.4Mbp的倒位。我们确定了额外的重排，包括相互易位和一个假定的环状染色体。有些缺失和倒位是通过生殖细胞传播的。
The maize Ac/Ds transposable element (TE) transposes by a "cut and paste'' mechanism. Previous studies in maize showed that when the TE ends are in reversed orientation with respect to each other, alternative transposition reactions can occur resulting in large scale genome rearrangements including deletions and inversions. To test whether similar genome rearrangements can also occur in other plants, we studied the efficacy of such alternative transposition-mediated genome rearrangements in Arabidopsis. Here we present our analysis of 33 independent chromosome rearrangements. Transposition at the reversed ends Ds element can cause deletions over 1 Mbp, and inversions up to 2.4 Mbp in size. We identified additional rearrangements including a reciprocal translocation and a putative ring chromosome. Some of the deletions and inversions are germinally transmitted.