Heterogeneity within MALDI samples as revealed by mass spectrometric imaging
Heterogeneity within MALDI samples as revealed by mass spectrometric imaging
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DOI：
10.1021/ac9908997
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发表时间：
2000-01-01
影响因子：
7.4
通讯作者：
Sweedler, JV
中科院分区：
文献类型：
--
作者：
Garden, RW;Sweedler, JV
虽然基质辅助激光解吸/电离质谱仪(MALDI MS)彻底改变了许多大分子的表征方式，但MALDI质谱图的外观高度多变仍然是一个令人担忧的问题，我们开发了基于MALDI的成像作为一种诊断工具，用于检查制备策略、样品形态和光谱质量之间的关系。成像协议包括自动获取单个样品中400-1600个位置的质谱图，然后进行离线处理和图像显示。已经对几种样品类型进行了表征，包括在干燥的2，5-二羟基苯甲酸液滴和α-氰基-4-羟基肉桂酸薄膜中制备的简单多肽混合物，以及由海洋软体动物Aplysia calfornica的完整多肽能神经元组成的复杂生物样品，成像实验提供了关于样品缺陷、光谱重复性、质量准确性、差异性分析物分布和内标有效性的丰富的无偏见信息。
While matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) has revolutionized the manner by which many large molecules are characterized, the highly variable appearance of MALDI mass spectra remains a concern, We have developed MALDI-based imaging as a diagnostic tool for examining the relationships between preparation strategy, sample morphology, and spectral quality. The imaging protocol involves the automated acquisition of mass spectra at 400-1600 positions within a single sample, followed by off-line processing and image display. Several sample types have been characterized, including a simple peptide mixture prepared in dried droplets of 2,5-dihydroxybenzoic acid and in thin films of alpha-cyano-4-hydroxycinnamic acid as well as a complex biological sample consisting of intact peptidergic neurons from the marine mollusk Aplysia californica, Imaging experiments provide a wealth of unbiased information concerning sample defects, spectral reproducibility, mass accuracy, differential analyte distributions, and the validity of internal standards.