Expression profiling reveals off-target gene regulation by RNAi
Expression profiling reveals off-target gene regulation by RNAi
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DOI：
10.1038/nbt831
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发表时间：
2003-06-01
影响因子：
46.9
通讯作者：
Linsley, PS
中科院分区：
文献类型：
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作者：
Jackson, AL;Bartz, SR;Linsley, PS
RNA干扰被认为需要小的干扰RNA（siRNA）与其同源mRNA之间的几乎认同。在这里，我们使用基因表达分析来表征siRNA在培养的人类细胞中基因沉默的特异性。转录曲线揭示了siRNA特异性的而不是靶标特异性特异性特征，包括直接沉默的非目标基因，这些基因含有与siRNA相同的11个连续核苷酸。这些结果表明，siRNA可能与有限序列相似性的靶标进行反应。
RNA interference is thought to require near-identity between the small interfering RNA (siRNA) and its cognate mRNA. Here, we used gene expression profiling to characterize the specificity of gene silencing by siRNAs in cultured human cells. Transcript profiles revealed siRNA-specific rather than target-specific signatures, including direct silencing of nontargeted genes containing as few as eleven contiguous nucleotides of identity to the siRNA. These results demonstrate that siRNAs may cross-react with targets of limited sequence similarity.