The MRL mouse repairs both cryogenic and ischemic myocardial infarcts with scar
The MRL mouse repairs both cryogenic and ischemic myocardial infarcts with scar
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DOI：
10.1016/j.carpath.2007.01.007
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发表时间：
2008-01-01
影响因子：
3.7
通讯作者：
Vassalli, Giuseppe
中科院分区：
文献类型：
--
作者：
Grisel, Philippe;Meinhardt, Andrea;Vassalli, Giuseppe
目的：MRL小鼠品系显示出非凡的伤口愈合能力。几年前，Leferovich等人（Proc Natl Acad Sci U. S. a. 2001; 98：9830 - 35）报道了在成年MRL小鼠中低温诱导的右心室心肌梗死后不存在瘢痕形成。一个独立的小组（Oh等人，2004; 13：203 - 6）发现，MRL小鼠在冠状动脉结扎后修复左心室缺血性梗塞，伴有规则的瘢痕形成。考虑到这两项研究报告的MRL小鼠梗死愈合的不同结果，我们研究了MRL小鼠在冷冻损伤和冠状动脉结扎模型中是否愈合了心肌梗死，而没有疤痕。方法和结果：在成年MRL和对照C57 Bl/6小鼠中测试了低温诱导的右心室和左心室损伤以及左冠状动脉前降支永久结扎的四种不同方案。在实验性梗死后60天，MRL小鼠显示受影响的右心室和左心室区域的明显瘢痕形成，使用五种实验方案中的任何一种，MRL和C57 B1/6小鼠之间的梗死大小和厚度没有显著差异。通过5-溴-2 '-脱氧尿苷（BrdU）掺入DNA中的细胞增殖分析未显示梗死后两种小鼠品系之间的任何差异。使用天狼星红染色的梗死区域的组织学分析没有显示两种小鼠品系之间胶原蛋白的程度和分布的差异。结论：NIRL小鼠通过对缺血性损伤和低温损伤的瘢痕形成来治愈心肌梗死。(c)2008年爱思唯尔公司All rights reserved.
Objective: The MRL mouse strain shows extraordinary wound healing capacities. Some years ago, Leferovich et al. (Proc Natl Acad Sci U. S. A. 2001;98:9830-35) have reported the absence of scar formation after cryogenically-induced right ventricular myocardial infarcts in adult MRL mice. An independent group (Oh et al., Cardiovasc Pathol 2004; 13:203-6) found that MRL mice repair left ventricular ischemic infarcts after coronary artery ligation with regular scar formation. Given the divergent outcomes in infarct healing in MRL mice reported by those two studies, we have investigated whether MRL mice heal myocardial infarcts, without scar both in the cryoinjury and in the coronary ligation model. Methods and Results: Four different protocols of cryogenically induced right and left ventricular injury, as well as permanent ligation of the left anterior descending coronary artery, were tested in adult MRL and control C57Bl/6 mice. At 60 days after experimental infarction, MRL mice showed pronounced scarring of the affected right and left ventricular areas, with no significant differences in infarct size and thickness between MRL and C57Bl/6 mice using any of the five experimental protocols. Analysis of cell proliferation by 5-bromo-2' -deoxyuridine (BrdU) incorporation into the DNA did not show any difference between the two strains of mice after infarction. Histological analysis of infarct areas using picrosirius red staining did not show differences in extent of collagen and distribution between the two mouse strains. Conclusions: NIRL mice heal myocardial infarcts with scar formation in response to ischemic as well as to cryogenic injuries. (c) 2008 Elsevier Inc. All rights reserved.