Tb(3+)-tRNA for LRET studies of protein synthesis.
Tb(3+)-tRNA for LRET studies of protein synthesis.
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Tb(3)-tRNA 用于蛋白质合成的 LRET 研究。
DOI：
10.1021/bc400062d
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发表时间：
2013
影响因子：
4.7
通讯作者：
Cooperman,BarryS
中科院分区：
文献类型：
--
作者：
Alonso,Dulce;Liu,Wei;Rosenblum,Gabriel;Mani,Tomoyasu;Goldman,YaleE;Cooperman,BarryS
当适当标记的大量tRNA转染到细胞中时，它们通过与核糖体结合产生FRET（荧光共振能量转移）信号，所述核糖体提供总蛋白质合成的测量。应用这种方法来监测特定蛋白质合成的速率需要实现非常高的信噪比。使用LRET（发光共振能量转移）代替FRET可以获得这样的高比率。含有天线发色团的镧系配合物是优良的LRET供体。在这里，我们描述了一个Phe-tRNAPhelelabeled与Tb 3+复合物，表示Tb 3 +-Phe-tRNAPhe，尽管庞大的Tb 3+复合物，是在蛋白质合成的活性的合成。
When suitably labeled bulk tRNAs are transfected into cells they give rise to FRET (fluorescence resonance energy transfer) signals via binding to ribosomes that provide a measure of total protein synthesis. Application of this approach to monitoring rates of specific protein synthesis requires achieving a very high signal-to-noise ratio. Such high ratios may be attainable using LRET (luminescence resonance energy transfer) in place of FRET. Lanthanide complexes containing an antenna chromophore are excellent LRET donors. Here we describe the synthesis of a Phe-tRNAPhelabeled with a Tb3+complex, denoted Tb3+-Phe-tRNAPhethat, notwithstanding the bulkiness of the Tb3+complex, is active in protein synthesis.