Maternal lymphocyte subpopulations (CD45RA+ and CD45RO+) in preeclampsia
Maternal lymphocyte subpopulations (CD45RA+ and CD45RO+) in preeclampsia
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DOI：
10.1067/mob.2002.127309
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发表时间：
2002-10-01
影响因子：
9.8
通讯作者：
Romero, R
中科院分区：
文献类型：
--
作者：
Chaiworapongsa, T;Gervasi, MT;Romero, R
目的：先兆子痫的母体综合征已被归因于全身性血管内炎症反应和内皮细胞功能障碍。在先兆子痫中，负责血管内炎症的刺激尚未确定。人T细胞表面上的CD45同种型的表达已被用于将CD4（+）T淋巴细胞分类为幼稚细胞（CD45RA+）和记忆T细胞（CD45RO+）。CD45RO+细胞百分比的增加被解释为与先前暴露于微生物产品或其他抗原一致。本研究的目的是确定先兆子痫是否与CD45RA+和CD45RO+比例的变化有关。在先兆子痫患者（n = 24）和正常妊娠患者（n = 75）中进行了前瞻性研究，采用流式细胞术和单克隆抗体检测外周血CD4（+）T淋巴细胞表面CD45RA+和CD45RO+的百分率。结果报告为CD4（+）淋巴细胞百分比。参数统计用于分析。的概率值
OBJECTIVE: The maternal syndrome of preeclampsia has been attributed to a systemic intravascular inflammatory response and endothelial cell dysfunction. The stimulus responsible for intravascular inflammation in preeclampsia has not been determined, The expression of CD45 isoforms on the surface of human T cells has been used to classify CD4(+) T lymphocytes into naive cells (CD45RA+) and memory T cells (CD45RO+). An increased percentage of CD45RO+ cells has been interpreted as consistent with previous exposure to microbial products or other antigens. The purpose of this study was to determine whether preeclampsia is associated with a change in the proportion of CD45RA+ and CD45RO+.STUDY DESIGN: A prospective study was conducted in patients with preeclampsia (n = 24) and normal pregnancy (n = 75), The percentage of CD45RA+ and CD45RO+ on CD4(+) T lymphocytes in peripheral blood was determined using flow cytometry and monoclonal antibodies. Results were reported as a percentage of CD4(+) lymphocytes. Parametric statistics were used for analysis. A probability value of