Synergistic, but not separate, stimulation of accumbal β1- and β2-adrenoceptors alters the accumbal dopamine efflux in freely moving rats
Synergistic, but not separate, stimulation of accumbal β1- and β2-adrenoceptors alters the accumbal dopamine efflux in freely moving rats
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DOI：
10.1016/j.ejphar.2013.04.035
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发表时间：
2013-09-05
影响因子：
5
通讯作者：
Cools, Alexander R.
中科院分区：
文献类型：
--
作者：
Aono, Yuri;Saigusa, Tadashi;Cools, Alexander R.
采用在体微透析技术，研究了脑内注射β-肾上腺素受体亚型选择性激动剂对自由活动大鼠延髓核去甲肾上腺素和多巴胺流出的影响。β（1）-（多巴酚丁胺：0.06和0.12 pmol）和β（2）-肾上腺素能受体激动剂（沙丁胺醇：0.36和3.6 pmol）都没有改变丘脑核中基础去甲肾上腺素和多巴胺的流出。0.06 pmol多巴酚丁胺与沙丁胺醇（3.6 pmol）联合给药不影响去甲肾上腺素水平，但可使多巴胺外排增加至约120%。0.12 pmol多巴酚丁胺与沙丁胺醇（0.36或3.6 pmol）联合给药也使DA外排增加至约120%，而不影响去甲肾上腺素水平。非选择性β-肾上腺素受体拮抗剂l-普萘洛尔（1200 pmol）不改变基础去甲肾上腺素和多巴胺水平，抑制多巴胺外排，由多巴酚丁胺（0.12 pmol）和沙丁胺醇（3.6 pmol）联合给药诱导。所述剂量是60分钟输注期内的药物总量。本实验结果支持了我们以前报道的结论，即刺激位于突触后的多巴胺能末梢的β-肾上腺素能受体，可以增强延髓核的多巴胺流出。本研究还提供了体内神经化学证据，即伴随而不是单独激活β（1-）和β（2-）肾上腺素受体协同增加多巴胺流出。(C)2013爱思唯尔有限公司版权所有。
The effects of intra-accumbal infusion of selective agonists for the beta-adrenoceptor subtypes on the noradrenaline and dopamine efflux in the nucleus accumbens of freely moving rats were investigated, using in vivo microdialysis. Neither beta(1)-(dobutamine: 0.06 and 0.12 pmol) nor beta(2)-adrenoceptor agonist (salbutamol: 0.36 and 3.6 pmol) altered the basal noradrenaline and dopamine efflux in the nucleus accumbens. Co-administration of 0.06 pmol of dobutamine with salbutamol (3.6 pmol) did not affect the noradrenaline levels, but it increased the dopamine efflux to approximately 120%. Co-administration of 0.12 pmol of dobutamine with salbutamol (0.36 or 3.6 pmol) also increased DA efflux to approximately 120% without affecting noradrenaline levels. The non-selective beta-adrenoceptor antagonist l-propranolol (1200 pmol) that did not alter the basal noradrenaline and dopamine levels, suppressed the dopamine efflux, induced by co-administration of dobutamine (0.12 pmol) and salbutamol (3.6 pmol). The doses mentioned are the total amount of drug over the 60-min infusion period. The present results support our previously reported conclusion that stimulation of accumbal beta-adrenoceptors which are suggested to be postsynaptically located on accumbal dopaminergic terminals, can enhance the dopamine efflux in the nucleus accumbens. The present study also provides in vivo neurochemical evidence that concomitant, but not separate, activation of accumbal beta(1-) and beta(2-)adrenoceptors synergistically increases the accumbal dopamine efflux. (C) 2013 Elsevier B.V. All rights reserved.