Transcriptome analysis of the synganglion from the honey bee mite, Varroa destructor and RNAi knockdown of neural peptide targets
Transcriptome analysis of the synganglion from the honey bee mite, Varroa destructor and RNAi knockdown of neural peptide targets
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DOI：
10.1016/j.ibmb.2015.12.007
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发表时间：
2016-03-01
影响因子：
3.8
通讯作者：
Bowman, Alan S.
中科院分区：
文献类型：
--
作者：
Campbell, Ewan M.;Budge, Giles E.;Bowman, Alan S.
瓦螨（Varroa destructor）及其传播的病毒是导致全球蜜蜂危机的主要因素之一。神经系统内的基因产物是目前用于控制瓦螨属的所有杀虫剂的靶标，但缺乏瓦螨属神经组织的转录组学数据。从成年雌性瓦螨的突触（“脑”）构建cDNA文库，并对600个EST进行测序和分析，揭示了几个当前和潜在的可药用靶标。编码变形翅病毒（DWV）变体V破坏者病毒-1（VDV-1）和重组体（VDV-1（DVD））的重叠群存在于突触神经节文库中。负义RNA特异性PCR表明，VDV-1复制在瓦螨synganglion和所有其他组织测试，但我们不能检测DWV复制在任何瓦螨组织。在synganlion EST库中鉴定了两种神经肽：B型allatostatin和甲壳动物高血糖激素（CHH）超家族的成员。通过双链RNA干扰（dsRNAi）敲低allatostatin或CHH样基因分别导致瓦螨属85%和55%的死亡率。在这里，我们提出了在瓦螨属的第一个转录组学调查，并证明了神经基因可以通过dsRNAi靶向，无论是在药物发现计划期间对推定靶点进行遗传验证，还是作为一种潜在的控制措施本身。(C)2016爱思唯尔有限公司版权所有
Varroa mites (Varroa destructor) and the viruses that they transmit are one of the major contributing factors to the global honey bee crisis. Gene products within the nervous system are the targets of all the insecticides currently used to control Varroa but there is a paucity of transcriptomic data available for Varroa neural tissues. A cDNA library from the synganglia ("brains") of adult female Varroa was constructed and 600 ESTs sequenced and analysed revealing several current and potential druggable targets. Contigs coding for the deformed wing virus (DWV) variants V destructor virus-1 (VDV-1) and the recombinant (VDV-1(DVD)) were present in the synganglion library. Negative-sense RNA-specific PCR indicated that VDV-1 replicates in the Varroa synganglion and all other tissues tested, but we could not detect DWV replicating in any Varroa tissue. Two neuropeptides were identified in the synganlion EST library: a B-type allatostatin and a member of the crustacean hyperglycaemic hormone (CHH) super family. Knockdown of the allatostatin or the CHH-like gene by double-stranded RNA-interference (dsRNAi) resulted in 85% and 55% mortality, respectively, of Varroa. Here, we present the first transcriptomic survey in Varroa and demonstrate that neural genes can be targeted by dsRNAi either for genetic validation of putative targets during drug discovery programmes or as a potential control measure in itself. (C) 2016 Elsevier Ltd. All rights reserved.