Selection and analysis of antigenic variants of the neuraminidase of N2 influenza viruses with monoclonal antibodies.
Selection and analysis of antigenic variants of the neuraminidase of N2 influenza viruses with monoclonal antibodies.
复制标题
用单克隆抗体选择和分析 N2 流感病毒神经氨酸酶抗原变异体。
DOI：
10.1016/0042-6822(82)90510-4
复制
发表时间：
1982
期刊：
影响因子：
3.7
通讯作者：
Laver,WG
中科院分区：
文献类型：
--
作者：
Webster,RG;Hinshaw,VS;Laver,WG
A/Tokyo/3/67流感病毒（H2N2）的神经氨酸酶（NA）的抗原变异体在NA单克隆抗体存在下病毒单次传代后以约1/105的频率被选择。用N2单克隆抗体对一组抗原变体进行分析，证明A/Tokyo/3/67的神经氨酸酶分子上至少有三个非重叠抗原区域，可能有四个。对野外毒株的分析表明，即使在新的大流行毒株出现后的第一年分离的流感病毒之间，神经氨酸酶的抗原变异也是明显的。A/Japan/305/57与A/Okuda/57和A/RI/5 +/57至少有7个表位不同。从1957年到1979年，人流感病毒神经氨酸酶的每个抗原区域都发生了抗原漂移，与早期毒株的单克隆抗体的反应性逐渐丧失，与后期毒株的抗体的反应性逐渐增强。在相似的时间段内，在禽类菌株的N2神经氨酸酶中仅检测到有限的抗原变异;所有禽类N2神经氨酸酶与A/Japan/305/57上的神经氨酸酶密切相关。血凝抑制单克隆抗体的NA发生在一些，但不是所有的N2病毒。这些结果表明，神经氨酸酶分子的分布可能不同的病毒拥有N2神经氨酸酶。
Antigenic variants of the neuraminidase (NA) of A/Tokyo/3/67 influenza virus (H2N2) were selected with a frequency of approximately 1 in 105after a single passage of the virus in the presence of monoclonal antibody to the NA. Analysis of a panel of antigenic variants with N2 monoclonal antibodies provided evidence for at least three nonover-lapping antigenic areas on the neuraminidase molecule of A/Tokyo/3/67 and possibly four. Analysis of field strains showed that antigenic variation in the neuraminidase was evident even between influenza virus isolated in the first year following the emergence of a new pandemic strain. A/Japan/305/57 differed in at least seven epitopes from A/ Okuda/57 and A/RI/5+/57. Antigenic drift occurred in each of the antigenic areas on the neuraminidase of human influenza virus from 1957 to 1979 with a gradual loss in reactivity with monoclonal antibodies to the early strains and acquisition of reactivity with antibodies to later strains. Only limited antigenic variation was detectable in the N2 neuraminidase for avian strains, over a similar time period; all of the avian N2 neuraminidases were closely related to the neuraminidase on A/Japan/305/57. Hemagglutination inhibition by monoclonal antibodies to the NA occurred in some but not all N2 viruses. These results suggest that the distribution of neuraminidase molecules may differ among viruses possessing N2 neuraminidase.