Muscle microvascular adaptation and angiogenic gene induction in response to exercise training are attenuated in middle-aged rats
Muscle microvascular adaptation and angiogenic gene induction in response to exercise training are attenuated in middle-aged rats
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DOI：
10.3920/cep150007
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发表时间：
2015-03
影响因子：
0.9
通讯作者：
J. Suzuki
中科院分区：
文献类型：
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作者：
J. Suzuki
本研究旨在探讨运动对中青年大鼠肌肉毛细血管化、血管生成基因mRNA表达和microRNA水平的影响。训练组的大鼠在跑步机上跑步，每周5天，持续3周。青年组（12周龄）运动方案为20-25米/分钟，40-60分钟/天，梯度为15%；中年组（12个月大）运动方案为18-20米/分钟，40-60分钟/天，梯度为5%。在腓肠肌横切面上进行了毛细管谱的酶组织化学鉴定。提取总RNA，进行逆转录，real-time PCR检测mRNA和microRNA水平。在年轻组中，运动训练显著提高了毛细血管与纤维的比率（提高了10%），但在中年组中仅略有提高（提高了5%）。急性运动后血管内皮生长因子（VEGF） mRNA水平显著升高（1.6倍）。
This study was designed to investigate exercise-induced changes in muscle capillarisation, the mRNA expression of angiogenic genes, and microRNA levels in young and middle-aged rats. Rats in the training groups were subjected to treadmill running 5 days a week for 3 weeks. The exercise protocol for the young (12-week old) group was 20-25 m/min, 40-60 min/day with a gradient of 15%, and for the middle-aged (12-month old) group was 18-20 m/min, 40-60 min/day with a gradient of 5%. The enzyme histochemical identification of capillary profiles was performed on cross-sections of gastrocnemius muscle. Total RNA was isolated, reverse transcription was performed, and mRNA and microRNA levels were determined by real-time PCR. The capillary-to-fibre ratio was significantly increased by exercise training in the young group (by 10%), but only slightly in the middle-aged (by 5%) group. Vascular endothecial growth factor (VEGF) mRNA levels were at significantly higher values after acute exercise (1.6-fold) and the 3-we...