Detection of lymphatic invasion in primary melanoma with monoclonal antibody D2-40 - A new selective immunohistochemical marker of lymphatic endothelium
Detection of lymphatic invasion in primary melanoma with monoclonal antibody D2-40 - A new selective immunohistochemical marker of lymphatic endothelium
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DOI：
10.1001/archderm.141.4.440
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发表时间：
2005-04-01
影响因子：
--
通讯作者：
From, L
中科院分区：
文献类型：
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作者：
Niakosari, F;Kahn, HJ;From, L
目的：使用单克隆抗体 D2-40（淋巴管内皮标记物）来识别原发性皮肤黑色素瘤中是否存在淋巴管浸润，并将淋巴管浸润的存在与肿瘤的其他临床病理特征相关联。设计：回顾性黑色素瘤病例系列研究，比较传统苏木精-伊红染色与 D2-40 免疫染色检测淋巴管浸润的效果。地点：病理学和皮肤科，多伦多大学桑尼布鲁克和女子学院健康科学中心，安大略省多伦多市。 I患者：连续检查44例肿瘤厚度大于0.75毫米的原发性皮肤黑色素瘤是否存在淋巴管侵犯。结果：44例黑色素瘤中的7例（16%）在D2-40免疫染色下显示存在淋巴管侵犯。 2例存在表皮下淋巴受累； 5例肿瘤内发现淋巴管侵犯，其中1例在肿瘤浸润边缘有额外的淋巴管侵犯。常规苏木精染色未检测到淋巴侵袭。我们观察到淋巴侵袭与肿瘤侵袭性的 2 个标志物之间的关联趋势，即较深的 Clark 水平和增加的溃疡频率，这表明用 D2-40 检测到的淋巴侵袭可能表明预后不良。结论：相对于传统的苏木精-伊红染色，在原发性黑色素瘤中，用 D2-40 免疫染色增加了检测到淋巴侵袭的频率。未来的结果数据将确定 D2-40 免疫染色检测到的淋巴侵袭的预后意义。
Objectives: To identify the presence of lymphatic invasion in primary cutaneous melanoma using monoclonal antibody D2-40, a marker of lymphatic endothelium, and to correlate the presence of lymphatic invasion with other clinicopathologic characteristics of the tumors.Design: Retrospective melanoma case series study comparing conventional hematoxylin-eosin staining with D2-40 inummostaining for detection of lymphatic invasion.Setting: Departments of Pathology and Dermatology, Sunnybrook and Women's College Health Sciences Center, University of Toronto, Toronto, Ontario. IPatients: Forty-four consecutive cases of primary cutaneous melanoma with a tumor thickness greater than 0.75 mm were examined for presence of lymphatic invasion.Results: Seven (16%) of 44 melanomas showed the presence of lymphatic invasion under immunostaining with D2-40. In 2 cases, subepidermal lymphatic involvement was present; in 5 cases lymphatic invasion was noted within the tumor, including I case of additional lymphatic invasion at the invasive edge of the tumor. Lymphatic invasion was not detected on routine hematoxylineosin staining. We observed a trend in the association between lymphatic invasion and 2 markers of tumor aggressiveness, namely a deeper Clark level and increased frequency of ulceration, which suggests that lymphatic invasion detected with D2-40 may indicate a poor prognosis.Conclusions: Immunostaining with D2-40 increases the frequency of detection of lymphatic invasion relative to conventional hematoxylin-eosin staining in primary melanoma. Future outcome data will determine the prognostic significance of lymphatic invasion detected by D2-40 immunostaining.