INDUCTION AND EXPRESSION OF HEPARIN-BINDING EGF-LIKE GROWTH-FACTOR IN HUMAN PANCREATIC-CANCER
INDUCTION AND EXPRESSION OF HEPARIN-BINDING EGF-LIKE GROWTH-FACTOR IN HUMAN PANCREATIC-CANCER
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DOI：
10.1006/bbrc.1994.2131
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发表时间：
1994-08-15
影响因子：
3.1
通讯作者：
KORC, M
中科院分区：
文献类型：
--
作者：
KOBRIN, MS;FUNATOMI, H;KORC, M
肝素结合EGF样生长因子（HB-EGF）是一种表观分子量为22千道顿的多肽，与表皮生长因子（EGF）有关，可结合并激活EGF受体。我们检测了HB-EGF在人胰腺癌细胞系中的生物学作用和表达，并比较了HB-EGF在正常胰腺组织和癌变胰腺组织中的表达。HB-EGF以剂量依赖的方式促进人胰腺癌细胞的生长。一些细胞系表达HB-EGF mRNA转录物，HB-EGF、12- o -十四烷酰基磷酸-13-醋酸酯和转化生长因子- α (tgf - α)可增强转录物水平。与正常胰腺相比，人胰腺癌组织中HB-EGF mRNA水平升高。这些发现提示HB-EGF可能参与EGF受体的异常自分泌和旁分泌激活，从而促进胰腺癌细胞的生长。(C) 1994学术出版社，Inc.
Heparin-binding EGF-like growth factor (HB-EGF) is a polypeptide with an apparent molecular weight of 22 kilodalton that is related to epidermal growth factor (EGF) and that binds and activates the EGF receptor. We examined HB-EGF biological action and expression in human pancreatic cancer cell lines, and compared HB-EGF expression in normal and cancerous pancreatic tissues. HB-EGF enhanced the growth of human pancreatic cancer cells in a dose-dependent manner. Several cell lines expressed HB-EGF mRNA transcripts, and the transcript level was enhanced by HB-EGF, as well as by 12-O-tetradecanoylphorbol-13-acetate and transforming growth factor-alpha (TGF-alpha). By comparison with the normal pancreas, HB-EGF mRNA levels were increased in human pancreatic cancer tissues. These findings suggest that HB-EGF may participate in aberrant autocrine and paracrine activation of the EGF receptor, thereby contributing to pancreatic cancer cell growth. (C) 1994 Academic Press, Inc.