ELECTRIC ACTIVITY OF CELLS IN THE EYE OF LIMULUS
ELECTRIC ACTIVITY OF CELLS IN THE EYE OF LIMULUS
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DOI：
10.1016/0002-9394(58)90800-6
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发表时间：
1958-01-01
影响因子：
4.2
通讯作者：
FUORTES, MGF
中科院分区：
文献类型：
--
作者：
FUORTES, MGF
METHODThe eye was dissected from the animal, cut in two along its longest diameter, and placed in artificial sea water. With the aid of a dis secting microscope individual ommatidia can be clearly seen in this preparation but the cells contained in them cannot be distin guished because of the black pigment sur rounding the ommatidia. The techniques used for stimulating and recording are similar to those employed in previous research (Frank and Fuortes, 1956) and will be described in detail elsewhere (Fuortes, 1958 b). It will be sufficient to mention here that a glass micropipette filled with potassium chloride was introduced into cells of the ommatidium and was used both for leading off the potentials generated across the cell's membrane during activity and for passing depolarizing or hyperpolarizing cur rents through the membrane of the cell. It should be noted that since these currents were passed through a bridge circuit (see Frank and Fuortes, 1956, fig. 1) the changes of membrane potential they evoked were not recorded but could be calculated if the values of current intensity and of membrane resistance were known.
