Biofabrication of stratified biofilm mimics for observation and control of bacterial signaling
Biofabrication of stratified biofilm mimics for observation and control of bacterial signaling
复制标题
DOI：
10.1016/j.biomaterials.2012.03.037
复制
发表时间：
2012-07-01
期刊：
影响因子：
14
通讯作者：
Bentley, William E.
中科院分区：
文献类型：
--
作者：
Luo, Xiaolong;Wu, Hsuan-Chen;Bentley, William E.
细胞间信号转导生物表型。个体细胞、细胞群和种群之间的通信存在于复杂的网络中，总而言之，这些网络指导着行为。很少有实验方法能够在与信号分子和细胞本身相称的长度和时间尺度上实现对个体和多细胞行为的高内容询问。在这里，我们提出的“生物制造”的微流体作为一种方法，使活细胞在微环境中的原位组织具有时空控制性和可编程性。我们构建了细菌生物膜模拟物，以一种与细胞数量分离的方式提供对基于群体的群体感应(QS)行为的详细理解和随后的控制。我们的方法揭示了单个生物膜内细菌细胞之间的信号模式，以及两个相互通信的生物膜之间协调的行为。我们展望了这种生物制造策略在细胞-细胞相互作用研究和小分子药物发现中的广泛应用。(C)2012爱思唯尔有限公司。保留所有权利。
Signaling between cells guides biological phenotype. Communications between individual cells, clusters of cells and populations exist in complex networks that, in sum, guide behavior. There are few experimental approaches that enable high content interrogation of individual and multicellular behaviors at length and time scales commensurate with the signal molecules and cells themselves. Here we present "biofabrication" in microfluidics as one approach that enables in-situ organization of living cells in microenvironments with spatiotemporal control and programmability. We construct bacterial biofilm mimics that offer detailed understanding and subsequent control of population-based quorum sensing (QS) behaviors in a manner decoupled from cell number. Our approach reveals signaling patterns among bacterial cells within a single biofilm as well as behaviors that are coordinated between two communicating biofilms. We envision versatile use of this biofabrication strategy for cell-cell interaction studies and small molecule drug discovery. (C) 2012 Elsevier Ltd. All rights reserved.