Centromere-Proximal Suppression of Meiotic Crossovers in Drosophila is Robust to Changes in Centromere Number and Repetitive DNA Content.
Centromere-Proximal Suppression of Meiotic Crossovers in Drosophila is Robust to Changes in Centromere Number and Repetitive DNA Content.
复制标题
果蝇减数分裂交叉的着丝粒近端抑制对于着丝粒数量和重复 DNA 含量的变化具有鲁棒性。
DOI：
10.1101/2023.10.17.562696
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Sekelsky,Jeff
中科院分区：
文献类型：
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作者：
Pazhayam,NilaM;Frazier,LeahK;Sekelsky,Jeff
减数分裂过程中同源染色体的准确分离取决于交换的存在和调节。着丝粒效应（CE），或在染色体的着丝粒周围区域的CO排斥，是一种减数分裂CO模式化现象，有助于防止不分离（NDJ），从而防止染色体疾病和其他减数分裂缺陷。尽管在近世纪前被发现，但这一基本细胞过程背后的机制仍然未知，大多数对果蝇CE的研究集中在着丝粒和着丝粒间异染色质的局部影响上。在这项研究中，我们试图调查是否剂量的变化，着丝粒数量和重复DNA含量的影响CE的强度，使用表型重组作图。此外，我们还研究了重复DNA功能CE强度的影响，使用卫星DNA结合蛋白突变体显示有缺陷的着丝粒聚类。尽管以前的研究表明，我们的研究结果表明，果蝇CE是强大的着丝粒数量和重复DNA含量的剂量变化，并可能也重复DNA功能。我们的研究表明，CE是不太可能的空间控制，提供新的洞察果蝇着丝粒效应背后的机制。
Accurate segregation of homologous chromosomes during meiosis depends on both the presence and regulated placement of crossovers (COs). The centromere effect (CE), or CO exclusion in pericentromeric regions of the chromosome, is a meiotic CO patterning phenomenon that helps prevent nondisjunction (NDJ), thereby protecting against chromosomal disorders and other meiotic defects. Despite being identified nearly a century ago, the mechanisms behind this fundamental cellular process remain unknown, with most studies of the Drosophila CE focusing on local influences of the centromere and pericentric heterochromatin. In this study, we sought to investigate whether dosage changes in centromere number and repetitive DNA content affect the strength of the CE, using phenotypic recombination mapping. Additionally, we also studied the effects of repetitive DNA function on CE strength using satellite-DNA binding protein mutants shown to have defective centromere clustering. Despite what previous studies suggest, our results show that the Drosophila CE is robust to dosage changes in centromere number and repetitive DNA content, and potentially also to repetitive DNA function. Our study suggests that the CE is unlikely to be spatially controlled, providing novel insight into the mechanisms behind the Drosophila centromere effect.