TLR9 signaling activation at different stages in colorectal cancer and NF-kappaB expression.
TLR9 signaling activation at different stages in colorectal cancer and NF-kappaB expression.
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DOI：
10.2147/ott.s174274
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发表时间：
2018
影响因子：
4
通讯作者：
Luo Y
中科院分区：
文献类型：
--
作者：
Gao C;Qiao T;Zhang B;Yuan S;Zhuang X;Luo Y
炎症与肿瘤的关系在结直肠癌发病机制的研究中变得越来越重要。 TLR9介导的免疫炎症反应在此过程中的作用目前尚不清楚。本研究旨在通过检测不同阶段结直肠癌组织中TLR9及其下游分子NF-κB的表达量,探讨TLR9信号激活与肿瘤进展的相关性。采用免疫组化链霉亲和素-过苷酶法和Western blot检测结直肠癌组织中TLR9的表达。结果显示，TLR9的高表达与肿瘤的低分化、侵袭、肝转移、血中CEA水平异常升高相关。随着信号激活，晚期结直肠癌中TLR9蛋白的水平比早期结直肠癌中升高更多。后来我们发现TLR9信号特异性表达的激活与组织学起源有关。 TLR9-C在晚期癌组织和癌旁组织中均有表达，TLR9-R蛋白在部分乙状结肠癌和直肠癌组织中占主导地位。随着TLR9的差异表达，其下游分子NF-κB蛋白的水平在结肠癌组织中升高，在直肠癌组织中降低。结果证实TLR9信号激活参与结直肠癌的临床过程并影响NF-κB的表达。
The relationship of inflammation and tumor is becoming more and more important in the study on the pathogenesis of colorectal cancer. The role of TLR9-mediated immune inflammation reaction in the process is not currently clear. The purpose of the study was to discuss the correlation of TLR9 signal activation with tumor progression by detecting the expression of TLR9 and its downstream molecule NF-kappaB in colorectal cancer tissues at different stages. TLR9 expression in colorectal cancer tissues was detected by immunohistochemical streptavidin-perosidase method and Western blot. The result showed that the high expression of TLR9 was correlated with tumor poorly differentiation, invasion and liver metastasis, the abnomal increasing levels of CEA in blood. With the signal activation, the levels of TLR9 protein raised more in advanced colorectal cancer than in early colorectal cancer. Afterward, we found that the activation of specific expression of TLR9 signal was related to histologic origin. TLR9-C expression displayed in both advanced cancer and para-carcinoma tissues, and TLR9-R protein was predominat in partial sigmoid and rectal cancer tissues. With the differential expression of TLR9, the levels of its downstream molecule NF-kappaB protein increased in colon cancer tissues and decreased in rectal cancer tissues. The results confirmed that TLR9 signaling activation participated in the clinical process of colorectal cancer and influenced NF-kappaB expression.