A Comprehensive Integrated Anatomical and Molecular Atlas of Rat Intrinsic Cardiac Nervous System
A Comprehensive Integrated Anatomical and Molecular Atlas of Rat Intrinsic Cardiac Nervous System
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DOI：
10.1016/j.isci.2020.101140
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发表时间：
2020-06-26
期刊：
影响因子：
5.8
通讯作者：
Schwaber, James S.
中科院分区：
文献类型：
--
作者：
Achanta, Sirisha;Gorky, Jonathan;Schwaber, James S.
我们开发并集成了多种技术，包括全器官成像和软件开发，以支持心内神经系统 (ICN) 的初始精确 3D 神经解剖图绘制和分子表型分析。虽然对 ICN 的解剖结构进行了定性和总体解剖学描述，但我们在这里以单细胞分辨率提供了整个大鼠 ICN 的综合图谱。我们的工作将解剖和分子数据精确地集成到 3D 数字重建的整个心脏中，分辨率达到微米级。现在，我们显示了大鼠心脏基部和左心房后部神经元簇的完整范围和位置，以及沿基部到心尖轴定义的分子表型的分布，这是以前未曾描述过的。这项工作所需的这些方法的发展已经产生了方法管道，为绘制其他器官提供了手段。
We have developed and integrated several technologies including whole-organ imaging and software development to support an initial precise 3D neuroanatomical mapping and molecular phenotyping of the intracardiac nervous system (ICN). While qualitative and gross anatomical descriptions of the anatomy of the ICN have each been pursued, we here bring forth a comprehensive atlas of the entire rat ICN at single-cell resolution. Our work precisely integrates anatomical and molecular data in the 3D digitally reconstructed whole heart with resolution at the micron scale. We now display the full extent and the position of neuronal clusters on the base and posterior left atrium of the rat heart, and the distribution of molecular phenotypes that are defined along the base-to-apex axis, which had not been previously described. The development of these approaches needed for this work has produced method pipelines that provide the means for mapping other organs.