Trapping of the malaria vector Anopheles gambiae with odour-baited MM-X traps in semi-field conditions in western Kenya
Trapping of the malaria vector Anopheles gambiae with odour-baited MM-X traps in semi-field conditions in western Kenya
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DOI：
10.1186/1475-2875-5-39
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发表时间：
2006-05-15
期刊：
影响因子：
3
通讯作者：
Knols, BGJ
中科院分区：
文献类型：
--
作者：
Njiru, BN;Mukabana, WR;Knols, BGJ
背景资料：气味诱饵诱捕系统的成功开发取决于行为活性化学信息素的识别，除了这种设备的设计和操作原理。在实验室调查中已经确定了各种各样的“引诱剂”，但其中很少有增加现场诱捕器捕获量的。一个包含的系统，中间的实验室和开放的领域，提出和以前的报告，人类的脚气味诱导冈比亚按蚊confirmed.Methods的行为反应：3 - 5日龄的女性的反应。gambiae朝向气味诱导的逆流几何捕集器（MM-X模型;美国生物物理公司，RI）在肯尼亚西部的半野外（屏蔽室）条件下进行了研究。陷阱用人脚气味（收集在袜子上）、二氧化碳（CO2，500 ml min（-1））、氨（NH3）、1-辛烯-3-醇或其各种组合作为诱饵。结果：除1-辛烯-3-醇外，所有气味诱饵均能显著（P < 0.05）增加诱捕器的捕获量。在尼龙或棉袜子织物上收集后，脚臭保持行为活跃至少8天。的协同反应观察到脚臭和CO2的组合（P < 0.001），其分别使单独这些气味的捕获量增加3.8倍和2.7倍。这些结果首次报告了非洲疟疾病媒在实验室外对人类脚臭的行为反应，需要进一步研究这种气味复合物的级分和/或单个化学组分。半场系统提供了候选利它素的高通量筛选的前景，这可能会加快开发有效的陷阱诱饵系统，这种和其他非洲蚊子物种。
Background: The successful development of odour-baited trapping systems for mosquitoes depends on the identification of behaviourally active semiochemicals, besides the design and operating principles of such devices. A large variety of 'attractants' has been identified in laboratory investigations, yet few of these increase trap catches in the field. A contained system, intermediate between the laboratory and open field, is presented and previous reports that human foot odour induces behavioural responses of Anopheles gambiae confirmed.Methods: The response of 3 - 5 day old female An. gambiae towards odour-baited counterflow geometry traps (MM-X model; American Biophysics Corp., RI) was studied in semi-field ( screen house) conditions in western Kenya. Traps were baited with human foot odour ( collected on socks), carbon dioxide (CO2, 500 ml min(-1)), ammonia (NH3), 1-octen-3-ol, or various combinations thereof. Trap catches were log (x+1) transformed and subjected to Latin square analysis of variance procedures.Results: Apart from 1-octen-3-ol, all odour baits caused significant ( P < 0.05) increases in trap catches over non-baited traps. Foot odour remained behaviourally active for at least 8 days after collection on nylon or cotton sock fabric. A synergistic response ( P < 0.001) was observed towards the combination of foot odour and CO2, which increased catches of these odours alone by 3.8 and 2.7 times, respectively.Conclusion: These results are the first to report behavioural responses of an African malaria vector to human foot odour outside the laboratory, and further investigation of fractions and/or individual chemical components of this odour complex are called for. Semi-field systems offer the prospect of high-throughput screening of candidate kairomones, which may expedite the development of efficient trap-bait systems for this and other African mosquito species.