Different Sequence Strains of Streptococcus agalactiae Elicit Various Levels of Cytokine Production
Different Sequence Strains of Streptococcus agalactiae Elicit Various Levels of Cytokine Production
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DOI：
10.1080/08820130802403283
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发表时间：
2008-01-01
影响因子：
2.8
通讯作者：
Manca, N.
中科院分区：
文献类型：
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作者：
De Francesco, M. A.;Gargiulo, F.;Manca, N.
B组链球菌(GBS)是新生儿和产科败血症最常见的原因，也是老年人和免疫功能低下患者败血症的日益重要的原因。我们的目的是评估不同的GBS基因型是否会诱导不同的促炎和抗炎细胞因子的产生。我们使用多位点序列分型方法对从无症状的健康携带者和有症状的个体中分离的71个克隆进行鉴定。用这些临床分离株感染纯化的人单核细胞。检测肿瘤坏死因子-α、白介素6、白介素8和白介素10的分泌。共鉴定出15个等位基因序列类型(STS)。MLST(多位点序列分型)分析将细菌分成四个不同的谱系(克隆簇)，其中两个与有症状的受试者的感染密切相关：CC17和CC19。此外，CC17和CC19对肿瘤坏死因子-6、IL-6和IL-8的刺激作用明显强于其他系，而CC17诱导的IL-10产生明显减少。这些结果表明，不同GBS基因型别感染的免疫反应存在差异。
Group B streptococcus (GBS) is the most common cause of neonatal and obstetric sepsis and an increasingly important cause of septicemia in elderly subjects and immunocompromised patients. Our aim was to evaluate whether different genotypes of GBS may induce a different production of pro-inflammatory and anti-inflammatory cytokines. We used multilocus sequence typing to identify 71 clones isolated from asymptomatic healthy carriers and symptomatic individuals. All these clinical isolates were used to infect purified human monocytes. TNF-alpha, IL-6, IL-8 and IL-10 secretion was measured. Fifteen allelic sequence types (STs) were identified. The MLST (multilocus sequence typing) anlaysis grouped the bacteria into four different lineages (clonal cluster) and two of these were closely involved in the infection of symptomatic subjects: CC17 and CC19. Furthermore, CC17 and CC19 stimulated TNF-, IL-6 and IL-8 production significantly more than the other lineages, while CC17 induced a decreased IL-10 production. These results suggest the existence of differences in immune response to infection with particular genotypes of GBS.