PRODUCTION OF HEAT-STABLE ENTEROTOXIN-II BY CHICKEN CLINICAL ISOLATES OF ESCHERICHIA-COLI
PRODUCTION OF HEAT-STABLE ENTEROTOXIN-II BY CHICKEN CLINICAL ISOLATES OF ESCHERICHIA-COLI
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DOI：
10.1016/0378-1097(93)90038-4
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发表时间：
1993-05-15
影响因子：
2.1
通讯作者：
OKAMOTO, K
中科院分区：
文献类型：
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作者：
AKASHI, N;HITOTSUBASHI, S;OKAMOTO, K
对从鸡腹泻粪便中分离的产肠毒素大肠杆菌进行了产热肠毒素II的检测，认为该毒素仅与猪腹泻有关。经菌落杂交和聚合酶链反应检测，38株大肠杆菌中有7株含有耐热肠毒素II基因。这些菌株的培养上清液在小鼠肠袢试验中引起液体蓄积。这种液体积累活性在100 ℃加热时没有丧失，并且被抗热稳定肠毒素II抗血清中和。纯化的热稳定肠毒素II引起鸡肠袢试验中的液体蓄积。这些结果表明，产生STII的E.大肠杆菌与鸡腹泻有关。
Enterotoxigenic Escherichia coli isolated from diarrhea stools of chickens were examined for production of heat-stable enterotoxin II which is considered to be implicated only in diarrhea of pigs. Seven out of 38 strains examined were found to contain heat-stable enterotoxin II gene, determined by colony hybridization and the polymerase chain reaction. The culture supernatants of these strains caused fluid accumulation in the mouse intestinal loop test. This fluid accumulation activity was not lost by heating at 100-degrees-C and was neutralized by anti-heat-stable enterotoxin II antiserum. Purified heat-stable enterotoxin II caused fluid accumulation in the chicken intestinal loop assay. These results indicate that STII-producing E. coli is implicated in chicken diarrhea.