Static compression induces zonal-specific changes in gene expression for extracellular matrix and cytoskeletal proteins in intervertebral disc cells in vitro
Static compression induces zonal-specific changes in gene expression for extracellular matrix and cytoskeletal proteins in intervertebral disc cells in vitro
复制标题
DOI：
10.1016/j.matbio.2003.11.008
复制
发表时间：
2004-01-01
期刊：
影响因子：
6.9
通讯作者：
Setton, LA
中科院分区：
文献类型：
--
作者：
Chen, J;Yan, W;Setton, LA
与椎间盘负重相关的压缩刺激被认为是椎间盘细胞代谢的重要调节因子。在本研究中，在海藻酸盐培养系统中，经过静态无侧限压缩（25%压缩应变）的不同时间段（2、18和30 h），定量了细胞外基质和细胞骨架蛋白基因表达水平的变化。在静态压缩基质蛋白后，观察到纤维环细胞和髓核细胞之间基因表达的差异。纤维环细胞在30h时间点对机械变形有反应，I型和II型胶原蛋白、聚集蛋白、大聚糖、decorin和lumican的基因表达增加。相比之下，髓核细胞对机械负荷没有反应，这些基质蛋白的基因表达在任何时候都发生了变化。我们的研究结果还表明，纤维环细胞，而不是髓核细胞，对静态压缩的反应是vimentin mRNA的表达增加以及vimentin亚基的聚合增加。目前的研究结果表明，纤维环的纤维软骨细胞可能在转录水平上调节海藻酸盐结构中机械变形后的生物合成。相反，髓核细胞对这些相同刺激的生物反应是无法检测到的。这些差异可能归因于未成熟髓核中脊索细胞群的存在，具有更分散和坚硬的细胞骨架，可以限制压缩载荷下的变形或形状变化。(C) 2003 Elsevier B.V./国际基质生物学学会。版权所有。
Compressive stimuli associated with weight-bearing and loading of the intervertebral disc are believed to be important regulators of disc cell metabolism. In this study, changes in gene expression levels for extracellular matrix and cytoskeletal proteins were quantified in disc cells in an alginate culture system subjected to static unconfined compression (25% compressive strain) after different time periods (2, 18 and 30 h). Differences in gene expression were observed between anulus fibrosus and nucleus pulposus cells following static compression for the matrix proteins studied here. Anulus fibrosus cells responded to mechanical deformation at the 30-h time point, with increasing gene expression for types I and II collagen, aggrecan, biglycan, decorin and lumican. In contrast, nucleus pulposus cells were not responsive to mechanical loading with changes in gene expression for these matrix proteins at any time. Our results also show that anulus fibrosus cells, but not nucleus pulposus cells, responded to static compression with increased expression of vimentin mRNA as well as increased polymerization of vimentin subunits. The results of the current study illustrate that fibrochondrocytes of the anulus fibrosus may regulate biosynthesis at the transcriptional level following mechanical deformation in an alginate construct. In contrast, the biological response of nucleus pulposus cells to these same stimuli is not detectable. These differences may be attributed to the presence of a notochordal cell population in the immature nucleus pulposus studied here, with a more diffuse and stiff cytoskeleton that may restrict deformations or shape changes upon compressive loading. (C) 2003 Elsevier B.V./International Society of Matrix Biology. All rights reserved.