CELL-CYCLE PARAMETERS OF ESCHERICHIA-COLI K-12
CELL-CYCLE PARAMETERS OF ESCHERICHIA-COLI K-12
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DOI：
10.1128/jb.173.24.7970-7974.1991
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发表时间：
1991-12-01
影响因子：
3.2
通讯作者：
BOYE, E
中科院分区：
文献类型：
--
作者：
ALLMAN, R;SCHJERVEN, T;BOYE, E
用计算机模拟程序计算了大肠杆菌K-12指数生长培养物的DNA分布。模拟的分布进行了比较，通过流式细胞仪实验获得的分布。通过最小化理论和实验DNA直方图之间的差异来确定DNA复制期（C）和复制后期（D）的持续时间。结果表明，K-12菌株AB 1157和CM 735的C和D周期彼此之间以及与先前测量的菌株B/rA的C和D周期之间存在很大差异，而菌株MC 1000显示出与菌株B/rA具有相同的C和D周期持续时间。K-12菌株之间的差异可以解释文献中关于它们的C和D周期的分歧。菌株W3110和AB 1157 recA 1的DNA直方图不能用经典的Cooper-Helmstetter模型充分模拟，这与W3110的起点和终点不对称以及AB 1157 recA 1的起始位置不对称一致。
A computer simulation routine was used to calculate the DNA distributions of exponentially growing cultures of Escherichia coli K-12. Simulated distributions were compared with distributions obtained experimentally by flow cytometry. Durations of the DNA replication period (C) and the postreplication period (D) were found by minimizing the difference between theoretical and experimental DNA histograms. It was demonstrated that the K-12 strains AB1157 and CM735 had C and D periods that differed widely from each other and from those of the previously measured strain B/rA, while strain MC1000 was shown to have the same durations of the C and D periods as strain B/rA. The variation between K-12 strains may explain the divergence in the literature regarding their C and D periods. Strains W3110 and AB1157 recA1 had DNA histograms that could not be adequately simulated by the classical Cooper-Helmstetter model, which is consistent with the asymmetrically located origin and terminus for W3110 and the asynchrony of initiation for AB1157 recA1.