STUDIES ON SEQUENCE OF 3'-TERMINAL REGION OF TURNIP-YELLOW-MOSAIC-VIRUS RNA
STUDIES ON SEQUENCE OF 3'-TERMINAL REGION OF TURNIP-YELLOW-MOSAIC-VIRUS RNA
复制标题
DOI：
10.1111/j.1432-1033.1977.tb11270.x
复制
发表时间：
1977-01-01
期刊：
影响因子：
--
通讯作者：
RAJBHANDARY, UL
中科院分区：
文献类型：
--
作者：
SILBERKLANG, M;PROCHIANTZ, A;RAJBHANDARY, UL
将完整的TYM病毒RNA与大肠杆菌RNase P一起温育后，纯化代表芜菁黄花叶(TYM)病毒RNA的3''-末端tRNA样区域的片段。该片段是112.+-。 3 个核苷酸长，用 T1 RNase 和胰腺 RNase 完全消化，并且使用 32P 末端标记技术在体外对此类消化物中存在的所有寡核苷酸进行测序。 TYM病毒RNA片段不含修饰核苷，并且不包含G-U-U-C-R序列。使用来自柑橘青霉的核酸酶 P1，推导出该片段的 5'' 末端 26 个核苷酸和 3'' 末端 16 个核苷酸的序列。 TYM病毒RNA片段5''末端的核苷酸序列表明该片段包括TYM病毒外壳蛋白基因的末端。
A fragment representing the 3''-terminal tRNA-like region of turnip yellow mosaic (TYM) virus RNA was purified following incubation of intact TYM virus RNA with Escherichia coli RNase P. This fragment, which is 112 .+-. 3-nucleotides long, was completely digested with T1 RNase and pancreatic RNase, and all the oligonucleotides present in such digests were sequenced using 32P-end labeling techniques in vitro. The TYM virus RNA fragment is free of modified nucleosides and does not contain a G-U-U-C-R sequence. Using nuclease P1 from Penicillium citrinum, the sequence of 26 nucleotides from the 5'' end and 16 nucleotides from the 3'' end of this fragment was deduced. The nucleotide sequence at the 5'' end of the TYM virus RNA fragment indicates that this fragment includes the end of the TYM virus coat protein gene.