Method for immobilizing microbial cells on gel surface for dynamic AFM studies
Method for immobilizing microbial cells on gel surface for dynamic AFM studies
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DOI：
10.1016/s0006-3495(95)80147-5
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发表时间：
1995-12-01
影响因子：
3.4
通讯作者：
Ikai, A
中科院分区：
文献类型：
--
作者：
Gad, M;Ikai, A
将酵母细胞轻轻地固定在3%琼脂上，然后浸泡在培养基中，用原子力显微镜成功地对酵母细胞的生长和出芽过程进行了6-7小时的成像。在化学固定细胞上进行的类似实验中，除了一开始的几次扫描外，没有发现任何明显的外观变化，这表明琼脂的溶解和/或扫描尖端刮擦其表面，如果有的话，并没有明显干扰之后拍摄的图像。许多未经处理的细胞高度的增加，伴随着它们的横向增大，被认为是酵母细胞生长过程的成功成像的指示，以及附着在母细胞上的正在生长的子细胞的图像。
The processes of cell growth and budding of the yeast cells Saccharomyces cerevisiae, which were gently immobilized on 3% agar and submerged in culture medium, were successfully imaged with an atomic force microscope for 6-7 h. Similar experiments on chemically fixed cells did not detect any appreciable change in their appearance except in a few scannings at the very beginning, indicating that the dissolution of agar and/or scraping of its surface by the scanning tip, if any, did not significantly interfere with the images taken thereafter. The increment in the height of many of the untreated cells, accompanied by their lateral enlargement, was taken as an indication of successful imaging of the growth process of yeast cells, together with an image of a growing daughter cell attached to its mother cell.