Mouse platelet-derived growth factor receptor alpha gene is deleted in W19H and patch mutations on chromosome 5.
Mouse platelet-derived growth factor receptor alpha gene is deleted in W19H and patch mutations on chromosome 5.
复制标题
小鼠血小板源性生长因子受体 α 基因在 W19H 中被删除，并在 5 号染色体上发生补丁突变。
DOI：
10.1073/pnas.88.11.4811
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发表时间：
1991
影响因子：
11.1
通讯作者：
Naylor,SL
中科院分区：
文献类型：
--
作者：
Smith,EA;Seldin,MF;Martinez,L;Watson,ML;Choudhury,GG;Lalley,PA;Pierce,J;Aaronson,S;Barker,J;Naylor,SL
小鼠W19 H突变是X射线诱导的5号染色体上超过2厘摩的缺失，包括白色斑点突变W（由Kit原癌基因编码）、斑块（Ph）和隐性致死（I）基因座。血小板衍生生长因子受体α基因（PDGFRA）样Kit编码跨膜受体酪氨酸激酶。利用小鼠-中国仓鼠体细胞杂交和种间回交小鼠单倍型分析，将小鼠Pdgfra定位于第5号染色体上，与Kit紧密连锁。PDGFRA探针与来自W19 H/ +杂合小鼠和斑片杂合小鼠及其野生型同窝小鼠的DNA的杂交证明了Pdgfra的缺失。脉冲场凝胶电泳表明Kit和Pdgfra连接在630-内切酶Mlu I DNA片段上。因此，W19 H缺失去除了至少两种受体酪氨酸激酶，并且结果表明Pdgfra作为Ph基因座的候选者。
The mouse W19H mutation is an x-ray-induced deletion of more than 2 centimorgans on chromosome 5 encompassing the white spotting mutation W (encoded by the Kit protooncogene), patch (Ph), and recessive lethal (l) loci. The platelet-derived growth factor receptor alpha gene (PDGFRA) like Kit encodes a transmembrane receptor tyrosine kinase. By using mouse-Chinese hamster somatic cell hybrids and haplotype analysis in interspecific backcross mice, mouse Pdgfra was mapped to chromosome 5 in tight linkage with Kit. Hybridization of a PDGFRA probe to DNAs from W19H/ + heterozygous mice and patch heterozygous mice, and their wild-type littermates, demonstrated deletion of Pdgfra. Pulsed-field gel electrophoresis indicated that Kit and Pdgfra are linked on a 630-kilobase Mlu I DNA fragment. Thus the W19H deletion removes at least two receptor tyrosine kinases and the results suggest Pdgfra as a candidate for the Ph locus.