Comparing the Chlorine Disinfection of Detached Biofilm Clusters with Those of Sessile Biofilms and Planktonic Cells in Single- and Dual-Species Cultures
Comparing the Chlorine Disinfection of Detached Biofilm Clusters with Those of Sessile Biofilms and Planktonic Cells in Single- and Dual-Species Cultures
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DOI：
10.1128/aem.05514-11
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发表时间：
2011-10-01
影响因子：
4.4
通讯作者：
Camper, Anne K.
中科院分区：
文献类型：
--
作者：
Behnke, Sabrina;Parker, Albert E.;Camper, Anne K.
尽管细胞从生物膜上的分离对于公共卫生和临床环境中的生物体传播至关重要，但尚未研究常用杀生物剂对分离的生物膜颗粒的消毒效果。因此，问题是分离的聚集体中的细胞是否可以用足以杀死细胞的消毒剂浓度杀死。洋葱伯克霍尔德菌和铜绿假单胞菌在标准化实验室反应器中作为单一菌种和共培养物生长。测量了恒化器和生物膜反应器流出物中的簇尺寸分布。氯敏感性进行了评估，浮游文化，附着的生物膜，颗粒和细胞脱离生物膜。消毒耐受性通常随着恒化器和分离的生物膜中较大细胞簇的百分比更高而增加。具有较低百分比的大簇的样品更容易消毒。因此，消毒耐受性取决于集群大小分布，而不是恒化器和分离的生物膜的样品类型。完整的生物膜更耐氯的物种无关。样品的均质化导致所有生物膜样品以及单个物种B的分离簇的敏感性显著增加。洋葱B. cepacia，B.共培养中的洋葱，和共培养中的铜绿假单胞菌。消毒效果也取决于物种组成;共培养有利于两个物种的生存时，生长为生物膜或集群从生物膜分离，但令人惊讶的是，导致在较低的消毒耐受性时，他们作为一个混合的反硝化培养生长。
Although the detachment of cells from biofilms is of fundamental importance to the dissemination of organisms in both public health and clinical settings, the disinfection efficacies of commonly used biocides on detached biofilm particles have not been investigated. Therefore, the question arises whether cells in detached aggregates can be killed with disinfectant concentrations sufficient to inactivate planktonic cells. Burkholderia cepacia and Pseudomonas aeruginosa were grown in standardized laboratory reactors as single species and in coculture. Cluster size distributions in chemostats and biofilm reactor effluent were measured. Chlorine susceptibility was assessed for planktonic cultures, attached biofilm, and particles and cells detached from the biofilm. Disinfection tolerance generally increased with a higher percentage of larger cell clusters in the chemostat and detached biofilm. Samples with a lower percentage of large clusters were more easily disinfected. Thus, disinfection tolerance depended on the cluster size distribution rather than sample type for chemostat and detached biofilm. Intact biofilms were more tolerant to chlorine independent of species. Homogenization of samples led to significantly increased susceptibility in all biofilm samples as well as detached clusters for single-species B. cepacia, B. cepacia in coculture, and P. aeruginosa in coculture. The disinfection efficacy was also dependent on species composition; coculture was advantageous to the survival of both species when grown as a biofilm or as clusters detached from biofilm but, surprisingly, resulted in a lower disinfection tolerance when they were grown as a mixed planktonic culture.