The identification of Cucumis sativus Glabrous 1 (CsGL1) required for the formation of trichomes uncovers a novel function for the homeodomain-leucine zipper I gene
The identification of Cucumis sativus Glabrous 1 (CsGL1) required for the formation of trichomes uncovers a novel function for the homeodomain-leucine zipper I gene
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DOI：
10.1093/jxb/erv046
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发表时间：
2015-05-01
影响因子：
6.9
通讯作者：
Ren, Zhonghai
中科院分区：
文献类型：
--
作者：
Li, Qiang;Cao, Chenxing;Ren, Zhonghai
对控制黄瓜毛状体发育的关键基因CsGL 1的研究揭示了HD-Zip I基因在植物中的新功能。果实是关系到果实市场价值的两个重要品质性状。然而，到目前为止，没有一个基因参与黄瓜果刺和开花毛状体的形成已被确定。在这里，野生型（WT）黄瓜和一个自发突变体，无毛1（csgl 1）由一个隐性核基因控制，与无毛的气生器官的毛状体发育的表征，报告。通过图位克隆，CsGL 1被分离，并发现它编码的同源域亮氨酸拉链I（HD-Zip I）蛋白的成员先前确定的功能主要在非生物胁迫反应的植物。组织特异性表达分析表明，CsGL 1在毛状体和果刺中强烈表达。另外，CsGL 1是一种具有弱转录激活活性的酵母核蛋白。对csgl 1和野生型黄瓜叶片的数字基因表达谱（DGE）分析表明，CsGL 1对黄瓜基因表达谱有显著影响，尤其是对细胞过程相关基因的影响，这与csgl 1和野生型黄瓜的表型差异相一致。此外，两个基因，CsMYB 6和CsGA 20 ox 1，可能参与黄瓜毛状体和果刺的形成，进行了表征。本研究揭示了HD-Zip I基因亚家族的新功能，为黄瓜果实外观品质的基因工程改良提供了候选基因。
The characterization of CsGL1, a key gene controlling the development of cucumber trichomes, reveals a new function for the HD-Zip I gene in plants.The spines and bloom of cucumber (Cucumis sativus L.) fruit are two important quality traits related to fruit market value. However, until now, none of the genes involved in the formation of cucumber fruit spines and bloom trichomes has been identified. Here, the characterization of trichome development in wild-type (WT) cucumber and a spontaneous mutant, glabrous 1 (csgl1) controlled by a single recessive nuclear gene, with glabrous aerial organs, is reported. Via map-based cloning, CsGL1 was isolated and it was found that it encoded a member of the homeodomain-leucine zipper I (HD-Zip I) proteins previously identified to function mainly in the abiotic stress responses of plants. Tissue-specific expression analysis indicated that CsGL1 was strongly expressed in trichomes and fruit spines. In addition, CsGL1 was a nuclear protein with weak transcriptional activation activity in yeast. A comparative analysis of the digital gene expression (DGE) profile between csgl1 and WT leaves revealed that CsGL1 had a significant influence on the gene expression profile in cucumber, especially on genes related to cellular process, which is consistent with the phenotypic difference between csgl1 and the WT. Moreover, two genes, CsMYB6 and CsGA20ox1, possibly involved in the formation of cucumber trichomes and fruit spines, were characterized. Overall, the findings reveal a new function for the HD-Zip I gene subfamily, and provide some candidate genes for genetic engineering approaches to improve cucumber fruit external quality.