Single-dose topical exposure to the pyrethroid insecticide, permethrin in C57BL/6N mice: effects on thymus and spleen.
Single-dose topical exposure to the pyrethroid insecticide, permethrin in C57BL/6N mice: effects on thymus and spleen.
复制标题
C57BL/6N 小鼠单剂量局部接触拟除虫菊酯杀虫剂氯菊酯：对胸腺和脾脏的影响。
DOI：
10.1016/s0278-6915(02)00163-1
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Holladay,SD
中科院分区：
文献类型：
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作者：
Prater,MR;GogalJr,RM;Blaylock,BL;Longstreth,J;Holladay,SD
在5周龄雌性C57 BL/6 N小鼠中评价了单次局部暴露于氯菊酯的免疫调节作用。暴露于5-25 μl氯菊酯的小鼠（相当于220-1100 mg/kg体重）对剃毛肩胛间皮肤的影响，评价体重变化;脾和胸腺器官重量和细胞结构;胸腺细胞表面表达、细胞凋亡;脾巨噬细胞吞噬作用和过氧化氢产生;脾B细胞抗体产生和T细胞溶细胞活性;和有丝分裂原诱导的脾细胞和胸腺细胞增殖后，在体内或体外氯菊酯暴露。局部使用二氯苯醚菊酯（25 μl）可导致脾T细胞增殖抑制32%;体外暴露于二氯苯醚菊酯也可使脾细胞增殖在25 μm和100 μm时分别减少72%和86%。氯菊酯似乎不影响其它白细胞功能测定。在暴露于15 μ l和25 μl二氯苯醚菊酯的小鼠中，分别观察到胸腺细胞构成的剂量相关性减少52%和80%。CD 4 −8−和CD 4 −8+胸腺细胞凋亡显著增加，CD 4 + CD 8+胸腺细胞亚群减少最严重，表明胸腺萎缩可能是化学诱导的凋亡机制。氯菊酯还导致脾细胞减少，15 μl时减少31%，25 μl时减少50%，这一效应可能与增殖抑制或细胞减少的胸腺接种减少有关。
Immunomodulatory effects of single topical exposure to permethrin were evaluated in 5-week-old female C57BL/6N mice. Mice exposed to 5–25 μl permethrin (equivalent to 220–1100 mg/kg body weight) on shaved interscapular skin were evaluated for altered body weight; splenic and thymic organ weight and cellularity; thymocyte cell surface expression, cellular apoptosis; splenic macrophage phagocytosis and hydrogen peroxide production; splenic B cell antibody production and T cell cytolytic activity; and mitogen-induced proliferation of splenocytes and thymocytes after in vivo or in vitro permethrin exposure. Topical permethrin application (25 μl) caused 32% inhibition of splenic T cell proliferation; in vitro exposure to permethrin also diminished splenocyte proliferation by 72% at 25 μm and 86% at 100 μm. permethrin did not appear to affect other leukocyte functional assays. Dose-related decreases in thymic cellularity of 52 and 80% were seen in mice exposed to 15 and 25 μl permethrin, respectively. Apoptosis was significantly increased in CD4−8−and CD4−8+thymocytes, and the CD4+CD8+thymocyte subpopulation was most severely diminished, suggesting possible chemical-induced apoptotic mechanism of thymic atrophy. Permethrin also caused splenic hypocellularity by 31% at 15 μl, and by 50% at 25 μl, an effect that may relate to inhibited proliferation or reduced seeding from the hypocellular thymus.