Silencing a sugar transporter gene reduces growth and fecundity in the brown planthopper, Nilaparvata lugens (Stål) (Hemiptera: Delphacidae).
Silencing a sugar transporter gene reduces growth and fecundity in the brown planthopper, Nilaparvata lugens (Stål) (Hemiptera: Delphacidae).
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DOI：
10.1038/srep12194
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发表时间：
2015-07-17
影响因子：
4.6
通讯作者：
Wu JC
中科院分区：
文献类型：
--
作者：
Ge LQ;Jiang YP;Xia T;Song QS;Stanley D;Kuai P;Lu XL;Yang GQ;Wu JC
褐飞虱(BPH)，褐飞虱，糖转运蛋白基因6(Nlst6)是一种在中肠表达的促进性葡萄糖/果糖转运蛋白(通常被称为被动载体)，介导糖从中肠腔到血淋巴的运输。糖转运蛋白基因表达下调对昆虫生长、发育和繁殖力的影响尚不清楚。尽管如此，我们有理由怀疑转运蛋白介导的食糖吸收对于以韧皮部为食的昆虫的生物学是必不可少的。基于这一推理，我们提出了一种假设，即抑制或减少BPH糖转运蛋白基因的表达将对昆虫有害。为了验证我们的假设，我们研究了Nlst6基因敲除对BPH生物学的影响。降低Nlst6的表达对BPHS有深远的影响。与对照组相比，显著延长了产卵前期，缩短了产卵期，减少了产卵量，减轻了体重。Nlst6基因敲除也显著降低了脂肪体和卵巢(尤其是卵黄蛋白原)的蛋白质含量以及卵黄蛋白原基因的表达。与对照组相比，实验性的BPHS积累的脂肪体内葡萄糖较少。推测Nlst6基因在褐飞虱的生长和繁殖中起作用，有可能成为防治韧皮部害虫的新靶标基因。
The brown planthopper (BPH), Nilaparvata lugens, sugar transporter gene 6 (Nlst6) is a facilitative glucose/fructose transporter (often called a passive carrier) expressed in midgut that mediates sugar transport from the midgut lumen to hemolymph. The influence of down regulating expression of sugar transporter genes on insect growth, development, and fecundity is unknown. Nonetheless, it is reasonable to suspect that transporter-mediated uptake of dietary sugar is essential to the biology of phloem-feeding insects. Based on this reasoning, we posed the hypothesis that silencing, or reducing expression, of a BPH sugar transporter gene would be deleterious to the insects. To test our hypothesis, we examined the effects of Nlst6 knockdown on BPH biology. Reducing expression of Nlst6 led to profound effects on BPHs. It significantly prolonged the pre-oviposition period, shortened the oviposition period, decreased the number of eggs deposited and reduced body weight, compared to controls. Nlst6 knockdown also significantly decreased fat body and ovarian (particularly vitellogenin) protein content as well as vitellogenin gene expression. Experimental BPHs accumulated less fat body glucose compared to controls. We infer that Nlst6 acts in BPH growth and fecundity, and has potential as a novel target gene for control of phloem-feeding pest insects.