Calcium phosphate particulates increase friction in the rat knee joint
Calcium phosphate particulates increase friction in the rat knee joint
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DOI：
10.1016/j.joca.2014.02.938
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发表时间：
2014-05-01
影响因子：
7
通讯作者：
Badger, G. J.
中科院分区：
文献类型：
--
作者：
Roemhildt, M. L.;Gardner-Morse, M. G.;Badger, G. J.
目的：碱性磷酸钙（BCP）颗粒通常存在于骨关节炎（OA）关节的软骨和滑液中，BCP的量与膝关节OA的严重程度相关。软骨矿化如何影响关节退行性变尚未确定。本研究的目的是确定是否BCP在滑液中影响大鼠膝关节摩擦系数（COF）。方法：从两个后肢的成年雄性大鼠4个膝关节的COF死后使用摆装置与红外跟踪系统测量。评价的三种条件是（1）初始状态，（2）注射100 μ L磷酸盐缓冲盐水（PBS）（假手术）后，和（3）注射100 μ L 1 mg/mL BCP悬浮液后。使用两种摩擦模型拟合摆幅的减小（衰减）：（1）单参数斯坦顿线性衰减模型和（2）两参数组合斯坦顿线性衰减和粘性阻尼指数衰减模型。注射BCP后COF较未注射BCP组增加17.6（P = 0.0012）和16.0%，与生理盐水注射（P = 0.0018）关节相比，来自单参数模型。COF在未处理关节和盐水注射关节之间没有差异。从两个参数模型的结果显示了类似的增加COF注射后的BCP，而粘性阻尼是没有显着不同的conditions.Conclusions：BCP颗粒的关节摩擦力增加表明BCP可能发挥作用，在关节面退化和OA的发展。(C)2014年国际骨关节炎研究学会。由爱思唯尔有限公司出版。保留所有权利。
Objective: Basic calcium phosphate (BCP) particulates are commonly found in cartilage and synovial fluid of osteoarthritis (OA) joints with the amount of BCP correlating with knee OA severity. How cartilage mineralization affects joint degeneration has yet to be determined. The objective of this study was to determine whether BCP in the synovial fluid affects the rat knee joint coefficient of friction (COF).Methods: The COFs of knees from both hind limbs of four mature male rats were measured post mortem using a pendulum apparatus with an infrared tracking system. The three conditions evaluated were (1) the naive state, (2) after the injection of 100 mu L of phosphate buffered saline (PBS) (sham) and (3) after the injection of 100 mu L of a 1 mg/mL BCP suspension. The decrease in the pendulum amplitude (decay) was fit using two friction models: (1) a one parameter Stanton linear decay model and (2) a two parameters combination Stanton linear decay and viscous damping exponential decay model.Results: The COF increased 17.6% after injection of BCP compared to the naive (P = 0.0012) and 16.0% compared to the saline injected (P = 0.0018) joints as derived from the one parameter model. The COF did not differ between naive and saline injected joints. Results from the two parameters model showed a similar increase in COF after injection of BCP while the viscous damping was not significantly different between conditions.Conclusions: The increased joint friction with BCP particulates suggests BCPs may play a role in articular surface degradation and OA development. (C) 2014 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.