Glycerolipidome responses to freezing- and chilling-induced injuries: examples in Arabidopsis and rice.
Glycerolipidome responses to freezing- and chilling-induced injuries: examples in Arabidopsis and rice.
复制标题
甘油脂组对冷冻和寒冷引起的损伤的反应：以拟南芥和水稻为例
DOI：
10.1186/s12870-016-0758-8
复制
发表时间：
2016-03-22
影响因子：
5.3
通讯作者：
Li W
中科院分区：
文献类型：
--
作者：
Zheng G;Li L;Li W
背景：甘油脂是细胞膜的主要成分；甘油脂的重塑在植物的温度适应中发挥着重要作用。温带植物可以承受冰冻胁迫，但即使零度以上的寒冷也会导致热带物种死亡。然而，人们对寒冷敏感植物和耐冻植物之间甘油脂对低温反应的差异知之甚少。使用基于 ESI-MS/MS 的脂质组学分析，我们比较了冷冻（4 和 10 °C）处理的水稻与冷冻（-6 和 -12 °C）处理的拟南芥的甘油脂组，无论是在这些低温处理之后还是在随后的恢复培养期之后。结果：拟南芥是一种 16:3 的植物，具有真核和原核型脂质合成途径，而水稻是一种18:3 植物仅具有真核脂质合成途径。拟南芥含有比水稻更高水平的半乳糖脂，并且具有更高的双键指数（DBI）。拟南芥含有较低水平的高熔点磷脂酰甘油 (PG) 分子，并且具有较低的平均酰基链长度 (ACL)。非致死冷冻处理的水稻在恢复培养期间发生了明显的磷脂降解，但在非致死冷冻处理的拟南芥中没有发生。在亚致死低温下，拟南芥中比水稻中合成更多具有较大头基的甘油脂。低温处理后，两种植物的磷脂酸（PA）和磷脂酰肌醇（PI）水平均上升。低温处理过程中总脂质的DBI和ACL没有变化。结论：较高的DBI和较低的ACL可以使拟南芥细胞膜在低温下更具流动性。合成含有较大头基的甘油脂的能力可能与低温耐受性相关。低温诱导的 PA 增加可能在植物对低温的反应中发挥双重作用：作为启动耐受反应的脂质信号，以及作为结构分子，在大量积累时可能会损害膜的完整性。 ACL和DBI的变化是植物对长期低温的反应。
Background:Glycerolipids are the principal constituent of cellular membranes; remodelling of glycerolipids plays important roles in temperature adaptation in plants. Temperate plants can endure freezing stress, but even chilling at above-zero temperatures can induce death in tropical species. However, little is known about the differences in glycerolipid response to low temperatures between chilling-sensitive and freezing-tolerant plants. Using ESI-MS/MS-based lipidomic analysis, we compared the glycerolipidome of chilling (4 and 10 °C)-treated rice with that of freezing (-6 and -12 °C)-treated Arabidopsis, both immediately after these low-temperature treatments and after a subsequent recovery culture period.Results:Arabidopsis is a 16:3 plant that harbours both eukaryotic and prokaryotic-type lipid synthesis pathways, while rice is an 18:3 plant that harbours only the eukaryotic lipid synthesis pathway. Arabidopsis contains higher levels of galactolipids than rice and has a higher double bond index (DBI). Arabidopsis contains lower levels of high melting point phosphatidylglycerol (PG) molecules and has a lower average acyl chain length (ACL). Marked phospholipid degradation occurred during the recovery culture period of non-lethal chilling treated rice, but did not occur in non-lethal freezing treated Arabidopsis. Glycerolipids with larger head groups were synthesized more in Arabidopsis than in rice at sub-lethal low-temperatures. Levels of phosphatidic acid (PA) and phosphatidylinositol (PI) rose in both plants after low-temperature treatment. The DBI and ACL of total lipids did not change during low-temperature treatment.Conclusions:A higher DBI and a lower ACL could make the membranes of Arabidopsis more fluid at low temperatures. The ability to synthesize glycerolipids containing a larger head group may correlate with low-temperature tolerance. The low-temperature-induced increase of PA may play a dual role in plant responses to low temperatures: as a lipid signal that initiates tolerance responses, and as a structural molecule that, on extensive in large accumulation, could damage the integrity of membranes. Changes in ACL and DBI are responses of plants to long-term low temperature.