Bioutilization of Cheese Whey and Corn Steep Liqour by Heterotrophic Microalgae Crypthecodinium cohnii for Biomass and Lipid Production
Bioutilization of Cheese Whey and Corn Steep Liqour by Heterotrophic Microalgae Crypthecodinium cohnii for Biomass and Lipid Production
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异养微藻 Crypthecodinium cohnii 对奶酪乳清和玉米浓液的生物利用用于生物质和脂质生产
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发表时间：
2017
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通讯作者：
M. Elibol
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作者：
Muge Isleten;M. Elibol
来自工业活动的许多不同的废水和副产品通过提供具有成本效益和可持续的解决方案而可能支持微藻生长。本研究以干酪乳清和玉米浆为主要原料，在异养微藻隐甲藻（Crypthecodinium cohnii）的培养基中进行微藻生物量和油脂生产的研究。确定了C. cohnii在用CSL制备的培养基中和在CW中生长，使用统计筛选工具。CSL显著促进微藻生长，可以替代酵母提取物作为主要营养源。CW是一种良好的C. cohnii补充了一些营养物质，并消除了对淡水的需求。因此，通过结合未稀释的CW和CSL开发了一种新的培养基，并对C.科尼。最后，在通过使用这种新培养基的放大尝试中，在3L搅拌罐生物反应器中进行微藻生产。C. cohnii在优化培养基中获得了较高的生物量生产率（2.28 g/L.d）和脂质含量（28.7%干重）。虽然C. cohnii因其积累大量二十二碳六烯酸（DHA）的能力而闻名，当其在主要补充有CSL的CW中培养时，其将其脂肪酸组成转化为增加比例的饱和和单不饱和脂肪酸（C16：0-C18：1），所述饱和和单不饱和脂肪酸（C16：0-C18：1）占总脂肪酸（TFA）的约70%。因此，C.当在这种新培养基中培养时，cohnii似乎比任何其他目的更适合于生物柴油的生产。
Many different wastewater and by-products derived from industrial activities potentially support microalgal growth by providing a cost-effective and sustainable solutions. In this present study, it was aimed to biologically utilize cheese whey (CW) and corn steep liquor (CSL) for microalgal biomass and lipid production by using these wastes in culture media for heterotrophic microalga Crypthecodinium cohnii cultivation. To determine nutrient requirements for C. cohnii growing in a medium prepared with CSL and in CW, statistical screening tools were used. CSL significantly enhanced microalgal growth and it could be an alternative to yeast extract as the primary nutrient source. As for CW, it served as a good culture medium for C. cohnii with the supplement of some of nutrients and eliminated the need for fresh water. Thus, a new culture medium was developed by combining undiluted CW and CSL and optimized for the growth of C. cohnii. Lastly, in a scale-up attempt by using this new medium, microalgal production was performed in a 3 L stirred tank bioreactor. C. cohnii yielded relatively high biomass productivity (2.28 g/L.d) and lipid content (28.7% dry weight) in the optimized medium. Although C. cohnii was known for its ability to accumulate high amounts of docosahexaenoic acid (DHA), it transformed its fatty acid composition to an increased proportion of saturated and monounsaturated fatty acids (C16:0-C18:1) that comprise ~70% of total fatty acids (TFA) when it was cultivated in CW mainly supplemented with CSL. Thus, C. cohnii seemed to be more feasible for biodiesel production than any other purposes when it was cultivated in this new medium.