Targeted reduction of oxytocin expression provides insights into its physiological roles.
Targeted reduction of oxytocin expression provides insights into its physiological roles.
复制标题
催产素表达的靶向减少提供了对其生理作用的深入了解。
DOI：
10.1007/978-1-4615-4871-3_30
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发表时间：
1998
影响因子：
--
通讯作者：
Wagner,KU
中科院分区：
文献类型：
--
作者：
Young3rd,WS;Shepard,E;DeVries,AC;Zimmer,A;LaMarca,ME;Ginns,EI;Amico,J;Nelson,RJ;Hennighausen,L;Wagner,KU
催产素是一种参与分娩和哺乳调节的九肽激素。它也与各种行为有关，如交配和母性，以及记忆。为了研究催产素（OT）是否对这些功能至关重要，我们通过同源重组消除了小鼠OT基因的大部分第一个内含子和最后两个外显子。这些外显子编码催产素前激素原的神经垂体素部分，假设其有助于OT的包装和运输。纯合子突变小鼠在室旁核、视上核或垂体后叶中没有可检测到的neurophysin或加工的催产素。有趣的是，纯合突变体雄性和雌性是可育的，纯合突变体雌性能够分娩它们的幼崽。然而，幼崽没有成功地消化，并在24小时内死亡，胃里没有牛奶。向母鼠注射OT或用大鼠OT基因拯救恢复了对吮吸的反应的乳汁排出。催产素也是产后肺泡增生所必需的。这些结果表明，催产素的绝对需要成功的牛奶喷射，但不是交配，分娩和牛奶生产，在小鼠。此外，纯合子突变小鼠在一些测试中表现出降低的攻击性。
Oxytocin is a nonapeptide hormone that participates in the regulation of parturition and lactation. It has also been implicated in various behaviors, such as mating and maternal, and memory. To investigate whether or not oxytocin (OT) is essential for any of thesefunctions, we eliminated, by homologous recombination, most of the first intron and the last two exons of the OT gene in mice. Those exons encode the neurophysin portion of the oxytocin preprohormone which is hypothesized to help in the packaging and transport of OT. The homozygous mutant mice have no detectable neurophysin or processed oxytocin in the paraventricular nucleus, supraoptic nucleus or posterior pituitary. Interestingly, homozygous mutant males and females are fertile and the homozygous mutant females are able to deliver their litters. However, the pups do not successfully suckle and die within 24 hours without milk in their stomachs. OT injection into the dams or rescue with the rat OT gene restores the milk ejection in response to suckling. OT is also needed for post-partum alveolar proliferation. These results indicate an absolute requirement for oxytocin for successful milk ejection, but not for mating, parturition and milk production, in mice. Furthermore, homozygous mutant mice show reduced aggression in some tests.