Studies on the isolation and identification of fetal nucleated red blood cells in the circulation of pregnant women before and after chorion villus sampling
Studies on the isolation and identification of fetal nucleated red blood cells in the circulation of pregnant women before and after chorion villus sampling
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DOI：
10.1159/000071983
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发表时间：
2003-09-01
影响因子：
2.2
通讯作者：
Philip, J
中科院分区：
文献类型：
--
作者：
Christensen, B;Kolvraa, S;Philip, J
目的：探讨不同分子形式的血红蛋白作为胎儿有核红细胞（NRBC）标志物的可行性。研究方法：在绒毛膜活检之前和之后，研究了纯胎儿血液和孕妇血液中是否存在γ-珠蛋白和γ-珠蛋白阳性NRBC。通过各种常规方法富集母体样品的NRBC，包括仅通过阳性CD 71选择或单一密度梯度进行有限富集。我们通过自动扫描在载玻片上寻找胎儿细胞。胎儿细胞的定义是（1）存在γ或γ球蛋白，（2）同时存在Y染色体信号。结果如下：绒毛膜活检后的25个血标本中，18个γ珠蛋白阳性细胞为Y染色体信号阳性，1个细胞有两个X染色体信号。从绒毛活检后的339例血标本中检出263例β珠蛋白阳性细胞，并与X、Y染色体探针杂交。无一例有两个X信号，249例Y阳性。在绒毛膜活检前的血标本中，仅发现1例胎儿NRBC阳性，未发现母血NRBC阳性。在绒毛膜活检前的12份血液样品中，仅在1份中鉴定出1个β珠蛋白阳性胎儿细胞，代表总共182 ml的母体血液。这表明，大多数胎儿细胞中发现的母体血液中的荧光原位杂交方法可能不是NRBC。版权所有（C）2003 S. Karger AG，巴塞尔。
Objective: To investigate the feasibility of various molecular forms of hemoglobin as markers for fetal nucleated red blood cells (NRBCs). Methods: The presence of epsilon and gamma globin positive NRBCs was investigated in pure fetal blood and in blood from pregnant women before and after chorion biopsy. Maternal samples were enriched for NRBCs by various conventional methods, including limited enrichment by only positive CD71 selection or single density gradient. We searched for fetal cells on slides by automated scanning. Fetal cells were defined by (1) the presence of epsilon or gamma globin and (2) simultaneously by the presence of a Y chromosome signal. Results: 18 of 25 gamma globin positive cells identified in blood samples after chorion biopsy were chromosome Y signal positive, and 1 cell had two X chromosome signals. 263 of 339 epsilon globin positive cells identified in blood samples after chorion biopsy were hybridized with X and Y chromosome probes. None had two X signals, and 249 were Y positive. In blood samples before chorion biopsy, only 1 epsilon globin positive fetal NRBC and no epsilon globin positive maternal NRBCs were found. Conclusions: Epsilon globin may be specific for fetal NRBCs. Only 1 epsilon globin positive fetal cell was identified in 1 of 12 blood samples before chorion biopsy, representing a total of 182 ml of maternal blood. This suggests that most fetal cells found in maternal blood by fluorescence in situ hybridization methods may not be NRBCs. Copyright (C) 2003 S. Karger AG, Basel.