Stimulation of macrophages by immunobiotic Lactobacillus strains: influence beyond the intestinal tract
Stimulation of macrophages by immunobiotic Lactobacillus strains: influence beyond the intestinal tract
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DOI：
10.1111/j.1348-0421.2012.00495.x
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发表时间：
2012-11-01
影响因子：
2.6
通讯作者：
Alvarez, Susana
中科院分区：
文献类型：
--
作者：
Marranzino, Gabriela;Villena, Julio;Alvarez, Susana
鼠李糖乳杆菌CRL1505 （Lr1505）、鼠李糖乳杆菌CRL1506 （Lr1506）和干酪乳杆菌CRL431 （Lc431）能够刺激肠道免疫，但只有Lr1505和Lc431能够刺激呼吸道免疫。为了进一步了解刺激远处粘膜部位的免疫机制，本研究评估了口服益生菌对肺泡和腹膜巨噬细胞功能的影响。与对照组相比，这三种乳酸菌均能显著提高腹腔巨噬细胞的吞噬和杀微生物活性。在腹腔内注射致病性白色念珠菌后，免疫生物制剂治疗的小鼠感染器官中的病原体计数明显降低。此外，乳杆菌处理的小鼠对白色念珠菌有更强的免疫反应。另一方面，只有Lc1505和Lc431能够提高肺泡巨噬细胞的活性和细胞因子的产生。只有这两组对白色念珠菌的呼吸挑战有更好的抵抗力，这与呼吸免疫反应的改善有关。本研究的结果表明，一些益生菌菌株的消耗可能有助于通过增加这些部位巨噬细胞的活性来提高对远离肠道部位感染的抵抗力。
Lactobacillus rhamnosus CRL1505 (Lr1505), L. rhamnosus CRL1506 (Lr1506) and L. casei CRL431 (Lc431) are able to stimulate intestinal immunity, but only Lr1505 and Lc431 are able to stimulate immunity in the respiratory tract. With the aim of advancing the understanding of the immunological mechanisms involved in stimulation of distant mucosal sites, this study evaluated the effects of orally administered probiotics on the functions of alveolar and peritoneal macrophages. Compared to a control group, these three lactobacilli were able to significantly increase phagocytic and microbicidal activities of peritoneal macrophages. After intraperitoneal challenge with pathogenic Candida albicans, mice treated with immunobiotics had significantly lower pathogen counts in infected organs. Moreover, lactobacilli-treated mice had a stronger immune response against C. albicans. On the other hand, only Lc1505 and Lc431 were able to improve activity of and cytokine production by alveolar macrophages. Only in these two groups was there better resistance to respiratory challenge with C. albicans, which correlated with improved respiratory immune response. The results of this study suggest that consumption of some probiotic strains could be useful for improving resistance to infections in sites distant from the gut by increasing the activity of macrophages at those sites.