MiR-483-5p downregulation contributed to cell proliferation, metastasis, and inflammation of clear cell renal cell carcinoma
MiR-483-5p downregulation contributed to cell proliferation, metastasis, and inflammation of clear cell renal cell carcinoma
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MiR-483-5p 下调导致透明细胞肾细胞癌的细胞增殖、转移和炎症
DOI：
10.1002/kjm2.12320
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发表时间：
2020-11-05
影响因子：
3.3
通讯作者：
Xiao, Wen
中科院分区：
文献类型：
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作者：
Wang, Xue-Gang;Zhu, Yong-Wu;Xiao, Wen
炎症状态对于肿瘤的生长尤其重要，而microRNAs(MiRNAs)被证实参与了肿瘤的发生和发展。然而，miR-483-5p在肾癌中的作用及其与炎症的关系尚未阐明。本研究旨在探讨miR-483-5p在肾透明细胞癌(CcRCC)中的表达及其与炎症状态的关系。应用基因芯片和实时定量聚合酶链式反应(qRT-PCR)技术，检测了miR-483-5p在血浆和肾细胞癌组织中的表达。分析miR-483-5p与肾细胞癌临床病理参数及炎症状态的相关性。用接收算子特征(ROC)曲线分析miR-483-5P的识别效率。体外实验探讨miR-483-5P在肾癌细胞中的生物学作用。术后第7天，5例基因芯片组和12例qRT-PCR组肾细胞癌患者血浆中MIR-483-5p表达上调。此外，58例肾细胞癌组织中miR-483-5p的表达低于癌旁组织。MIR-483-5P能较好地区分肾细胞癌和正常组织，其曲线下面积(AUC)为0.739(P<0.0001)。MiR-483-5p高表达与肿瘤分期呈正相关，而miR-483-5p相对高表达与中性粒细胞/淋巴细胞比率(NLR)(P=0.03)和淋巴细胞/单核细胞比率(LMR)(P=0.026)呈负相关。MiR-483-5p过表达可逆转上皮-间充质转化(EMT)过程，抑制肾癌细胞增殖和转移。我们的发现提示miR-483-5p的表达与炎症状态呈负相关，可能是ccRCC的一个潜在的血浆生物标志物。
Inflammation status are especially for tumor growth, and microRNAs (miRNAs) confirmed to participate in cancer occurrence and progression. However, the role of miR-483-5p and the relation with inflammation have not been elucidated in renal cell cancer (RCC). In this study, we intended to explore miR-483-5p expression and the relationship of inflammation status in clear cell renal cell cancer (ccRCC). Using microarray and qRT-PCR (Quantitative Real-time Polymerase Chain Reaction), we investigated the miR-483-5p expression in plasma and ccRCC cancer tissues. Then, we analyzed the correlation of miR-483-5p with clinicopathological parameters and inflammation status in ccRCC. Receiver operator characteristic (ROC) curves analysis was used to analyze the discrimination efficiency of miR-483-5p. in vitro experiments explored the biological role of miR-483-5p in renal cancer cells. miR-483-5p expression was upregulated in plasma of 5 patients with microarray and 12 patients with qRT-PCR in ccRCC at day 7 postoperatively. In addition, low expression of miR-483-5p was found in 58 ccRCC cancer tissues when compared with non-cancerous tissues. miR-483-5p could sufficiently discriminate ccRCC with the area under the curve (AUC) of 0.739 (P < .0001) from normal tissues. Higher expression of miR-483-5p was positively related to lower tumor stage and higher relative expression of miR-483-5p was inversely related to neutrophil-to-lymphocyte ratio (NLR) (P = .03) and lymphocyte-to-monocyte ratio (LMR) (P = .026). Overexpression of miR-483-5p lead to reverse epithelial-mesenchymal transition (EMT) process, restrain cell proliferation and metastasis of renal cancer cells. Our findings suggest that miR-483-5p expression is negatively correlation with inflammation status and may be a potential plasma biomarker for ccRCC.